For mouse tissue inflammatory mediator quantification, corneal epithelial and conjunctival samples were removed following experimental conditions (6 mice per sample, n = 3 experiments) and pooled tissue was homogenized in 0.2% Triton X-100/PBS containing a protease inhibitor cocktail (Roche, Nutley, NJ, USA). MMPs (MMP-2, MMP-3, MMP-8, MMP-9, and MMP-12) and cytokines/chemokines (IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12, IL-13, IL-15, IL-17, IP-10, MKC, MCP-1, MIP-1α, MIP-1β, MIP-2, G-CSF, GM-CSF, IFNγ, TNFα, CXCL1, and RANTES) were quantitated using MILLIPLEX MAP Mouse MMP Magnetic Bead Panel 3 and Cytokine/Chemokine Panel- Immunology Multiplex Assays (EMD Millipore, San Diego, CA, USA), per manufacturer's instructions. Total protein concentrations were determined by Direct Detect Assay Kit (EMD Millipore) and 10 μg of total protein was loaded per well in duplicate.