Purchase this article with an account.
Max Villain, Françoise Sandillon, Agnès Muller, Emmanuel Candon, Gérard Alonso, Bernard Arnaud, Alain Privat; Macroglial Alterations after Isolated Optic Nerve Sheath Fenestration in Rabbit. Invest. Ophthalmol. Vis. Sci. 2002;43(1):120-128.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. To study the modifications undergone by the macroglial cells after
meningeal breach of the optic nerve in the rabbit, without optic
methods. The optic nerve sheath fenestration technique carried out in humans was
adapted to rabbit without axonal injury in the optic nerve. The effects
of meningeal fenestration on glial cells were examined by
immunocytochemical procedures (day 15) using the antibodies against two
astrocyte markers: glial fibrillary acidic protein (GFAP) and vimentin.
Proliferation of glial cells was evaluated with single
5-bromodeoxyuridine (BrdU) labeling or double GFAP and BrdU labelings.
Qualitative data on glial cells were evaluated with the electron
results. Optic nerve sheath fenestration on healthy adult rabbits resulted in a
decrease of volume of the subarachnoid space located at the level of
the meningeal scar, with a significant increase of the optic nerve
area. The meninges presented a fibrous scar. In the optic nerve
parenchyma, astrocytes appeared hypertrophic in the vicinity of the
fenestration. The whole nerve contains numerous BrdU-labeled mitotic
cells, a number of which double-labeled for both BrdU and GFAP belong
to the astrocyte line. There was no loss of optic nerve axons.
conclusions. The inflammation produced by the surgical breach of the peri-optic
meningeal sheaths induces a significant reactivity, including
proliferation of astrocytes in the optic nerve. Reactive astrocytes may
interact positively with axons and may modify the extracellular
environment in the optic nerve.
This PDF is available to Subscribers Only