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Angela F. Drew, Heidi L. Schiman, Keith W. Kombrinck, Thomas H. Bugge, Jay L. Degen, Adam H. Kaufman; Persistent Corneal Haze after Excimer Laser Photokeratectomy in Plasminogen-Deficient Mice. Invest. Ophthalmol. Vis. Sci. 2000;41(1):67-72.
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purpose. Excimer laser photorefractive keratectomy creates a nonvascular wound
of the cornea. Fibrin deposition and resolution after excimer laser
photokeratectomy were investigated in relation to corneal repair and
restoration of clarity in mice with a genetic deficiency of
methods. A Summit Apex Laser (Summit, Waltham, MA) was used to perform
2-mm, 175-pulse, transepithelial photoablations that resulted in deep
stromal keratectomies. Photokeratectomy was performed on the corneas of
plasminogen-deficient (Plg−/−) mice and
littermate control animals. Eyes were examined for re-epithelialization
and clarity throughout the 21-day observational period. Histologic
sections were taken during the observational period and fibrin(ogen)
was detected immunohistochemically.
results. Re-epithelialization was rapid and complete within 3 days in both
control andPlg−/− animals. Exuberant corneal
fibrin(ogen) deposition was noted in Plg−/− mice and
sparse fibrin(ogen) deposition in control mice on days 1 and 3 after
injury. Fibrin(ogen) deposits resolved in control mice but persisted in
Plg−/− mice (74% of eyes at 21 days; P < 0.004). Corneal opacification, scarring, and
the presence of anterior chamber fibrin(ogen) occurred in
plasminogen-deficient mice but not in control mice.
conclusions. Fibrin(ogen) deposition occurs during corneal wound repair after
photokeratectomy. Impaired fibrinolysis in Plg−/− mice
caused persistent stromal fibrin deposits that correlated with the
development of corneal opacity.
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