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Tamar Masos, Jacob A. Dan, Ruth Miskin; Plasminogen Activator Inhibitor-1 mRNA Is Localized in the Ciliary Epithelium of the Rodent Eye. Invest. Ophthalmol. Vis. Sci. 2000;41(5):1006-1011.
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purpose. To identify in the adult and developing rodent eye cells expressing the
gene encoding plasminogen activator inhibitor-1 (PAI-1), an important
component of the fibrinolytic system.
methods. PAI-1 mRNA was localized in cryostat thin eye sections via in situ
hybridization analysis using specific 35S-labeled
riboprobes. PAI-1 activity was tested in the aqueous humor using
one-phase reverse zymography.
results. In the adult eye, PAI-1 mRNA was detected exclusively in epithelial
cells of the ciliary processes, primarily in the apexes. In addition,
PAI-1 activity was detected in the aqueous humor. PAI-1 mRNA was first
found in the ciliary epithelium in embryonic day 18.5, when the ciliary
body has reached an advanced developmental stage. PAI-1 mRNA was also
detected in the ganglion cell layer of the retina at postnatal days 1
to 4, when angiogenesis takes place.
conclusions. During development, PAI-1 is likely to be involved in retina
vascularization, in agreement with other cases of angiogenesis. Results
for the adult eye indicate that the ciliary epithelium is the source
for PAI-1 activity found in the aqueous humor. The results suggest that
PAI-1 plays a role in balancing fibrinolysis and proteolysis
specifically in the anterior segment of the eye, implying that PAI-1
overproduction in the ciliary epithelium could shift the balance
against proteolysis and thus may interfere with aqueous
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