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Lavinia Taliana, Margaret D. M. Evans, Slobodan D. Dimitrijevich, John G. Steele; Vitronectin or Fibronectin Is Required for Corneal Fibroblast–Seeded Collagen Gel Contraction. Invest. Ophthalmol. Vis. Sci. 2000;41(1):103-109.
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purpose. The wound healing process in the corneal stroma involves the activation
of corneal keratocytes and the expression of associated phenotypes
(fibroblasts and myofibroblasts). One of these phenotypes, the
myofibroblasts, synthesizes α-smooth muscle actin in order to affect
wound closure by contracting the surrounding matrix. Excessive
contraction results in the formation of unresolvable scars that are
undesirable in the corneal stroma. The authors tested the effect of
vitronectin and fibronectin on the contraction process associated with
corneal wound healing.
methods. Collagen gels were prepared and were exposed to different treatments
of fetal calf serum (FCS). The FCS used was either depleted of
fibronectin and vitronectin or contained a known concentration of
fibronectin, vitronectin, or both at 50 μg/ml. Contraction was
measured using image analysis and cross sections of contracted gels
were examined for α-smooth muscle actin expression using laser
results. Fibroblasts seeded in collagen gels paralleled the morphologic
characteristics and cell distribution of keratocytes in unwounded
cornea. Matrix contraction was dependent on the presence of fibronectin
and/or vitronectin where myofibroblasts were present. The cell-mediated
contraction process was maximal at 0.5 × 105 fibroblasts/ml.
conclusions. These studies showed that vitronectin or fibronectin is required for
the myofibroblast-associated contraction to occur in this in vitro
model of stromal wound healing. This model system shows a distinct
potential for further studies relating to the corneal wound healing
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