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Marc D. de Smet, Molly Dayan; Prospective Determination of T-Cell Responses to S-Antigen in Behçet’s Disease Patients and Controls. Invest. Ophthalmol. Vis. Sci. 2000;41(11):3480-3484.
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purpose. To prospectively determine, using two different assays, the lymphocyte
proliferative response to a retinal autoantigen (S-antigen) in patients
with Behçet’s disease who are under treatment for ocular
methods. Patients were evaluated at each visit for signs of ocular inflammation.
Peripheral blood leukocytes were harvested and cultured in the presence
of bovine S-antigen in a standard culture assay, as well as by limiting
dilution using multiple short-term T-cell lines.
results. Five patients were observed for 2 to 10 months. During follow-up, three
patients had episodes of ocular inflammation. No consistent change in
proliferative response was observed in standard proliferation assays.
However, an increase in established T-cell lines was correlated to the
presence of ocular inflammation in all three patients. Ocular activity
was associated with an increase of 9- to 30-fold in the frequency of
short-term T-cell lines. This increase returned to baseline within 1 to
conclusions. An increase in S-antigen–responsive lymphocytes is found in the
peripheral blood of patients with Behçet’s disease during
episodes of ocular inflammation. This increase cannot be measured using
standard proliferation assays but requires the use of techniques
exploiting the principles of limiting dilution
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