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Kjell Johansson, Anitha Bruun, Jan deVente, Berndt Ehinger; Immunohistochemical Analysis of the Developing Inner Plexiform Layer in Postnatal Rat Retina. Invest. Ophthalmol. Vis. Sci. 2000;41(1):305-313.
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purpose. To investigate the development from early postnatal life to adulthood
of neural cell processes that establish the circuitry of the inner
plexiform layer (IPL). Emphasis was focused on the ontogeny of subsets
of cGMP- and protein kinase C (PKC)-immunoreactive amacrine and bipolar
methods. Paraformaldehyde-fixed postnatal and adult retinas were used for light
microscopic analysis of immunohistochemical labeling of cryo-sections.
Synthesis of cGMP in neural structures was achieved by means of an in
vitro stimulation with a well-established nitric oxide donor.
results. In vitro stimulation of postnatal and mature retina with the nitric
oxide donor results in NO-activated cGMP synthesis in subsets of
bipolar and amacrine cells. NO-activated cGMP immunoreactivity is
expressed in specific cell populations during the first postnatal week.
Other cell subsets, consisting of amacrine cells and rod bipolar cells,
express PKC immunoreactivity during postnatal development. An
increasing number of rod bipolar cells start to exhibit cGMP labeling
after eye opening, and a colocalization with PKC is established in
adult retinas. Processes from these cell populations terminate in
several sublaminas in the developing IPL, but cGMP- and PKC-labeled
terminals appear to be confined to ON-lamina as the retina matures.
conclusions. The development of cGMP- and PKC-labeled fibers within the IPL appears
to be in concert with events of neural differentiation and
synaptogenesis. These results suggest that the nitric oxide/cGMP
signaling pathway and PKC may participate in activity-dependent
processes during development that establish the mature circuitry of
synaptic contacts within the IPL. The presence of cGMP in mature rod
bipolar cells suggests a role in the signal transduction of rod bipolar
cell–AII amacrine cell pathway.
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