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Qian Garrett, Bronwyn Laycock, R. Wayne Garrett; Hydrogel Lens Monomer Constituents Modulate Protein Sorption. Invest. Ophthalmol. Vis. Sci. 2000;41(7):1687-1695.
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© ARVO (1962-2015); The Authors (2016-present)
purpose. To examine the effect of hydrogel lens monomer constituents on protein
methods. A series of hydroxyethylmethacrylate (HEMA)-based hydrogels with
various amounts of methacrylic acid (MAA) or N-vinyl pyrrolidone
(NVP) were synthesized. A radiolabel tracer technique was used to
measure the amount of protein adsorbed on or penetrating into the
hydrogels. Penetration of fluorescence-labeled proteins in the
hydrogels was studied by laser scanning confocal microscopy.
Single-protein solutions of human serum albumin (HSA) and hen egg
lysozyme were studied.
results. Inclusion of the comonomers MAA or NVP in hydrogels resulted in an
increase in water content and also had a strong impact on protein
sorption. An increase in the amount of MAA in the
poly(HEMA-co-MAA) hydrogels increased lysozyme adsorption
and penetration but reduced HSA adsorption. However, the amount of
protein adsorbed for both HSA and lysozyme increased with the amount of
NVP in the poly(HEMA-co-NVP) hydrogels. In contrast to the
marked effect of MAA on protein sorption, in particular, on lysozyme
sorption, NVP had little influence on protein sorption. When a hydrogel
contains both MAA and NVP, MAA has the dominant effect on protein
sorption—in particular, on lysozyme sorption. Furthermore, a large
difference was observed in the amount of lysozyme adsorbed on the
hydrogels that had similar water contents but little variation in
adsorption of HSA.
conclusions. Negatively charged carboxyl groups of the MAA constituent may influence
lysozyme sorption in two ways: by electrostatic attraction and by
increasing the possibility for the small lysozyme molecule to penetrate
the hydrogels. Interactions of the surface lactam groups of NVP with
proteins may be attributable to the attraction of proteins to NVP.
Water content is not a primary factor in determining protein
adsorption. It appears that the monomer constituents, such as MAA or
NVP, control protein adsorption.
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