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Mark I. Rosenblatt, Gerhard P. Dahl, Ian M. Dickerson; Characterization and Localization of the Rabbit Ocular Calcitonin Gene-Related Peptide (CGRP)-Receptor Component Protein (RCP). Invest. Ophthalmol. Vis. Sci. 2000;41(5):1159-1167.
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purpose. To determine whether the calcitonin gene-related peptide (CGRP)
receptor component protein (RCP), a novel signal transduction molecule,
is required for CGRP signaling in the eye and to determine potential
ocular sites of CGRP action.
methods. The cDNA for the rabbit ocular RCP homologue was cloned using a
combination of reverse transcription-polymerase chain reaction (RT-PCR)
and rapid amplification of cDNA ends (RACE). Function of the rabbit
ocular RCP was assessed using a sensitive oocyte-based assay, which
utilizes the protein kinase A (PKA)-sensitive cystic fibrosis
transmembrane conductance regulator (CFTR) as a sensor of cAMP
formation. RCP expression in the rabbit eye was localized using
results. A 2063-bp cDNA for the rabbit ocular RCP was cloned and sequenced.
Expression of the rabbit RCP cDNA confers CGRP responsiveness in a
sensitive oocyte-based assay. Antisense oligonucleotides made to the
ocular RCP abolishes CGRP responsiveness of ciliary body and iris mRNA
in the oocyte–CFTR assay. Localization of RCP protein in the rabbit
eye using immunohistochemistry demonstrated RCP immunoreactivity in the
ciliary body and iris blood vessels, as well as in layers of the
conclusions. The rabbit ocular RCP appears to be required for signal transduction at
ocular CGRP receptors and is localized to sites previously reported to
bind CGRP, which affect intraocular pressure and neurogenic
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