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Thomas Desmettre, Claude-Alain Maurage, Serge Mordon; Heat Shock Protein Hyperexpression on Chorioretinal Layers after Transpupillary Thermotherapy. Invest. Ophthalmol. Vis. Sci. 2001;42(12):2976-2980.
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purpose. To assess a biological effect induced by temperature elevation during
transpupillary thermotherapy (TTT).
methods. Six pigmented rabbits were anesthetized, and TTT was performed on the
right eye using an 810-nm diode laser installed on a slit lamp (spot
size, 1.3 mm; duration, 60 seconds; power, 92–150 mW). A series of
laser pulses were aimed at the posterior pole of the retina. The left
eyes were used as the control. Twenty-four hours after laser
irradiation, a histologic study was performed on the chorioretinal
layers. Tissue samples were fixed in formalin and embedded in paraffin.
A monoclonal antibody was used to detect heat shock protein (Hsp)70
immunoreactivity, followed by a biotinylated goat anti-mouse antibody,
revealed by the avidin-biotin complex and the 3-amino-9-ethyl-carbazole
(AEC) chromogen. Retinal structures were further identified by
hematoxylin erythrosin saffron (HES) coloration.
results. The photocoagulation threshold was found to be at the 150-mW laser
power. Under this threshold, Hsp70 immunostaining was the strongest at
the 127-mW power, with staining of some choroidal cells, including
capillary endothelial cells. No Hsp70 immunoreactivity was observed on
the retina. At the 107-mW power, Hsp70 reactivity was observed only in
occasional choroidal cells. At the 98-mW power, only mild, diffuse
Hsp70 immunoreactivity was observed in the choroid. At the 92-mW power,
as in nonirradiated eyes, no Hsp70 immunoreactivity was detected.
conclusions. Subthreshold transpupillary 810-nm laser irradiation induces choroidal
Hsp hyperexpression. This confirms that choroidal Hsp hyperexpression
can be induced during TTT, as has been recently hypothesized by several
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