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Geoffrey P. Lewis, Steven K. Fisher; Müller Cell Outgrowth after Retinal Detachment: Association with Cone Photoreceptors. Invest. Ophthalmol. Vis. Sci. 2000;41(6):1542-1545.
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purpose. Subretinal gliosis is a relatively common occurrence after retinal
reattachment. Because Müller cell processes only intermittently
penetrate the outer limiting membrane (OLM) beneath extensive
detachments, this study was conducted to determine whether this was
preferentially associated with rod or cone photoreceptors.
methods. Cat retinas were detached from the retinal pigment epithelium and 3
days later were fixed in 4% paraformaldehyde, embedded in 5% agarose,
sectioned at 100 μm, and processed for standard immuohistochemistry.
The retinas were double labeled with either anti-vimentin and
anti-long/medium wavelength–sensitive (anti-L/M) cone opsin or
anti-glial fibrillary acidic protein (GFAP) and biotinylated peanut
results. The hypertrophy of Müller cells was readily traced using
antibodies to vimentin and GFAP. When labeling with these antibodies
was combined with labeling by either antibodies to cone opsins or
biotinylated PNA, a consistent relationship was observed between the
Müller cell processes growing through the OLM and cone
conclusions. The growth of Müller cell processes into the subretinal space
forms a fibrotic layer that completely inhibits the regeneration of
outer segments. The current results show that there appears to be a
highly specific interaction between growing Müller cell processes
and cone photoreceptors during the earliest phase in this
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