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Lee P. Schelonka, David Siegel, Matthew W. Wilson, Alex Meininger, David Ross; Immunohistochemical Localization of NQO1 in Epithelial Dysplasia and Neoplasia and in Donor Eyes. Invest. Ophthalmol. Vis. Sci. 2000;41(7):1617-1622.
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purpose. To examine the expression of NAD(P)H:quinone oxidoreductase 1 (NQO1,
DT-diaphorase), a potential bioactivating enzyme for mitomycin C in
corneal and conjunctival epithelial dysplasia and neoplasia and in
normal tissues from human donor eyes, by immunohistochemistry.
methods. Formalin-fixed, paraffin-embedded sections of human donor eyes and
tissue sections with histologic diagnoses of corneal and conjunctival
epithelial dysplasia and neoplasia from the Eye Pathology Laboratory,
University of Colorado Health Sciences Center were analyzed. Detection
of NQO1 in tissues was performed using standard immunohistochemical
techniques with monoclonal antibodies against NQO1 and immunoperoxidase
results. All 20 tumors stained positive for NQO1. In seven eyes from four
donors, positive staining for NQO1 was detected in all epithelial and
endothelial layers, in fibroblasts, in all retinal layers except the
photoreceptor outer segments, and in the fascicles and arachnoid of the
optic nerve. Only minimal staining was detected in the photoreceptor
outer segments and the optic nerve pia and dura. Immunostaining was
markedly reduced in all tissues in both eyes from donor 5. Genetic
analysis confirmed that this individual was homozygous for a
polymorphism in NQO1 (NQO1*2).
conclusions. NQO1 was detected by immunohistochemistry in every examined section of
corneal and conjunctival epithelial dysplasia and neoplasia, suggesting
that NQO1 may play a role in the bioactivation of mitomycin C in these
tumors. However, the presence of NQO1 in the corneal, conjunctival, and
ciliary epithelium; the retinas; and the optic nerves of donor eyes may
indicate the potential for mitomycin C toxicity, particularly at higher
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