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Tetsuya Sugiyama, Hidehiro Oku, Shoichiro Ikari, Tsunehiko Ikeda; Effect of Nitric Oxide Synthase Inhibitor on Optic Nerve Head Circulation in Conscious Rabbits. Invest. Ophthalmol. Vis. Sci. 2000;41(5):1149-1152.
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purpose. To study the effect of a nitric oxide synthase inhibitor on tissue
circulation in the optic nerve head (ONH) of conscious rabbits.
methods. N G-nitro-l-arginine methyl ester
(l-NAME) (1, 10, or 100 mg/kg), d-NAME (10
mg/kg), or physiological saline was administered intravenously to
albino rabbits. A quantitative index of blood velocity, the normalized
blur (NB), was measured in the ONH by laser speckle tissue circulation
analyzer. The intraocular pressure (IOP) and blood pressure (BP) were
also measured. l-arginine (10 mg/kg) was intravenously
administered 20 minutes after l-NAME (10 mg/kg) injection.
Acetylcholine (ACh; 10 μg/kg per minute) was infused for 15 minutes,
with or without pretreatment of l-NAME (1 mg/kg).
results. l-NAME induced a continuous decrease of the NB in a
dose-dependent manner, but d-NAME caused no significant
change. At 100 mg/kg, l-NAME significantly increased the
IOP, mean BP, and ocular perfusion pressure, but the other doses caused
no significant changes. When l-arginine was administered
after l-NAME injection, the NB returned to its initial
level and remained there. Pretreatment with l-NAME
inhibited the increase of NB induced by ACh.
conclusions. These results indicate that nitric oxide regulates basal tissue
circulation in the ONH of conscious rabbits and suggest that ACh
increases the circulation by promoting nitric oxide
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