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Amund Ringvold, Erlend Anderssen, Inge Kjønniksen; Distribution of Ascorbate in the Anterior Bovine Eye. Invest. Ophthalmol. Vis. Sci. 2000;41(1):20-23.
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purpose. To analyze the ascorbate distribution in the anterior eye wall to
better understand the functional significance of this compound in the
methods. Ascorbic acid was determined by high-performance liquid chromatography
using an LC-10 system (Shimadzu, Kyoto, Japan). Bovine eye samples were
results. The highest ascorbate concentration was observed in the corneal
epithelium, with significantly higher values in the central (1.56 mg/g)
than in the peripheral (1.39 mg/g) area. The ascorbate content was
similar in the corneal stroma (0.22 mg/g), the Descemet’s membrane
(DM)/endothelium (0.22 mg/g), and the aqueous humor (0.21 mg/ml). By
comparison, the sclera (0.15 mg/g) and the conjunctiva (0.11 mg/g)
showed lower values, as did the lacrimal gland (0.09 mg/g) and the
serum (0.0008 mg/ml).
conclusions. (1) Peak ascorbate concentration was observed in the central corneal
epithelium covering the pupillary area. This is compatible with the
idea that the ascorbate may act as an UV filter shielding internal eye
structures from radiation damage. (2) The ascorbate concentration in
the corneal stroma and DM/endothelium was as high as in the aqueous
humor, and it is suggested that the aqueous humor plays a key role in
the distribution of ascorbate to the anterior eye
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