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Makoto Aihara, James D. Lindsey, Robert N. Weinreb; Reduction of Intraocular Pressure in Mouse Eyes Treated with Latanoprost. Invest. Ophthalmol. Vis. Sci. 2002;43(1):146-150.
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© 2015 Association for Research in Vision and Ophthalmology.
purpose. To determine whether topical treatment of mouse eyes with latanoprost
alters intraocular pressure (IOP).
methods. In a masked study design, NIH Swiss mice received a 2-μL topical
instillation of 0.00015%, 0.0006%, 0.0025%, or 0.01% latanoprost or
vehicle (phosphate-buffered saline [PBS]). After 1, 2, or 3 hours,
the animals were anesthetized, and a fluid-filled glass microneedle
connected to a pressure transducer was inserted through the cornea into
the anterior chamber to measure IOP. The reduction of IOP after
latanoprost measurement was calculated by the comparison between
treated and nontreated eyes in the same mouse. The effect of
latanoprost after a single 0.01% dose was also measured at 6, 12, and
24 hours. As in the previous study, the identity of all eye drop
solutions was masked.
results. In mouse eyes receiving topical PBS, the mean IOP was 14.8 ± 2.2
mm Hg (n = 173 males). There was no significant
difference in IOP between male and female eyes and between right and
left eyes. At 1 hour after topical treatment with 0.00015% or 0.0025%
latanoprost, IOP increased by as much as 11% ± 7%. At 2 and at 3
hours after application, IOP decreased in a dose-dependent manner.
These decreases were significant in eyes receiving 0.0025% or 0.01%
latanoprost (P < 0.05, Student-Newman-Keuls test)
and the largest decrease (14% ± 8%) was noted 2 hours after
treatment with 0.01% latanoprost. At 6, 12, or 24 hours after
treatment, there was no difference in latanoprost- and PBS-treated
conclusions. Latanoprost reduces mouse IOP in a dose-dependent manner. The mouse may
be a useful model for studying the effect of drugs on
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