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WeiYan Zhang, John Hawse, QingLing Huang, Nancy Sheets, Kevin M. Miller, Joseph Horwitz, Marc Kantorow; Decreased Expression of Ribosomal Proteins in Human Age-Related Cataract. Invest. Ophthalmol. Vis. Sci. 2002;43(1):198-204.
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purpose. To identify lens epithelial genes with altered expression levels in
age-related human cataract.
methods. Epithelia from age-related cataracts and from normal lenses were
microdissected and RNA was extracted. RNAs were compared for gene
expression differences by RT-PCR differential display. Transcripts
exhibiting altered levels of gene expression were cloned and identified
by sequencing. The expression levels of identified clones were
confirmed by semiquantitative RT-PCR with three separately isolated RNA
preparations. Specific primers were designed and used to examine the
mRNA levels of other genes important in protein synthesis.
results. Numerous transcripts exhibited altered levels of gene expression. One
transcript exhibiting a decreased level of expression in cataract
compared with normal lenses was identified as encoding ribosomal
protein L21. Three additional ribosomal proteins, L15, L13a, and L7a,
also exhibited decreased expression in cataract compared with normal
human lenses. By contrast, the levels of elongation factor (EF)-1α1
and eucaryotic initiation factor (eIF)-4E remained unchanged.
conclusions. The results provide evidence that human age-related cataract is
associated with decreased expression of L21 and other ribosomal
proteins. The results suggest that modulation of protein synthesis
and/or other functions mediated by ribosomal proteins is associated
with age-related cataract.
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