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H. Monique H. Hurks, Markus M. Valter, Louis Wilson, Ivan Hilgert, Peter J. van den Elsen, Martine J. Jager; Uveal Melanoma: No Expression of HLA-G. Invest. Ophthalmol. Vis. Sci. 2001;42(13):3081-3084.
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© ARVO (1962-2015); The Authors (2016-present)
purpose. To investigate whether uveal melanoma cells express HLA-G, a
nonclassical HLA class I molecule that has been shown to be a critical
mediator in the inhibition of natural killer (NK) cell–mediated
methods. Eleven human uveal melanoma cell lines were analyzed for the expression
of HLA-G by flow cytometry, immunocytochemistry, Western blot analysis,
and RT-PCR followed by Southern blot analysis. Two HLA-G–specific
monoclonal antibodies were used, 87G and MEM-G/1. In addition, HLA-G
expression was determined on frozen tissue sections of 17 primary uveal
results. With all HLA-G detection methods, no evidence for HLA-G expression by
uveal melanoma cells was found. In contrast, the trophoblast cell line
JEG-3 clearly expressed HLA-G transcripts and protein in all cases.
Furthermore, interferon-γ did not induce HLA-G expression in the
uveal melanoma cell lines. Notably, all cell lines expressed HLA-E, and
this expression was significantly enhanced by interferon-γ.
conclusions. Because none of the uveal melanoma cell lines nor any of the primary
uveal melanomas displayed expression of HLA-G, it is unlikely that
HLA-G plays a role, direct or indirect, in the modulation of cellular
immunity against uveal melanoma tumors.
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