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Dody Avichezer, Phyllis B. Silver, Chi-Chao Chan, Barbara Wiggert, Rachel R. Caspi; Identification of a New Epitope of Human IRBP that Induces Autoimmune Uveoretinitis in Mice of the H-2b Haplotype. Invest. Ophthalmol. Vis. Sci. 2000;41(1):127-131.
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purpose. Experimental autoimmune uveoretinitis (EAU) is a T-cell–mediated
disease induced by immunization with interphotoreceptor retinoid
binding protein (IRBP). Major uveitogenic sites have been identified
for mice of the H-2r and H-2k haplotypes but
not for the H-2b haplotype. The present
communication describes the characterization of an epitope contained in
residues 1 to 20 of human IRBP that induces EAU in H-2b mice.
methods. H-2b (C57BL/6, 129/J) and H-2r (B10.RIII) mice
were immunized with peptide 1-20 or with whole (bovine) IRBP. EAU
(histopathology) and immunologic responses (delayed-type
hypersensitivity [DTH], lymphocyte proliferation, and cytokine
production) were assessed after 21 days.
results. C57BL/6 mice, 129/J and (129/JxC57BL/6)F1 mice, immunized with 200 to
300 μg of peptide, developed DTH and EAU with scores comparable to
those induced by 100 μg IRBP. Their lymphocytes proliferated to the
peptide and produced interferon-γ (but not interleukin-4) and
transferred EAU to syngeneic recipients. Lymphocytes of IRBP-immunized
mice also responded to the peptide. Peptide 1-20–immunized B10.RIII
mice failed to develop either disease or immunologic responses.
conclusions. Human IRBP peptide 1-20 contains a major epitope for the
H-2b haplotype, which is apparently not presented by the
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