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Wakako Adachi, Kousaku Okubo, Shigeru Kinoshita; Human Uroplakin Ib in Ocular Surface Epithelium. Invest. Ophthalmol. Vis. Sci. 2000;41(10):2900-2905.
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purpose. To investigate the expression and localization of the human gene
encoding uroplakin Ib in ocular surface epithelium.
methods. The full-length cDNA of human uroplakin Ib was isolated from a cDNA
library of human corneal epithelium, and the expression of uroplakin Ib
in various tissues was examined by reverse transcription–polymerase
chain reaction (RT-PCR). In cornea and conjunctiva, the expressions of
uroplakin Ia, II, and III were also examined by RT-PCR. Finally, the
localization of uroplakin Ib in the ocular surface was analyzed by
immunofluorescence confocal microscopy, by using an antiserum against a
results. Two mRNA isoforms, arising through two polyadenylation sites, were
isolated. RT-PCR detected uroplakin Ib in cornea, conjunctiva, bladder,
placenta, and kidney. Among other uroplakins, uroplakin II was also
faintly detected in cornea and conjunctiva. Immunofluorescence confocal
microscopy documented uroplakin Ib protein in the cell membranes of
superficial and wing cells in the corneal epithelium. It was not found,
however, in the most apical corneal epithelial cells. In limbus and
conjunctiva, uroplakin Ib was also localized in the cell membranes of
all epithelial layers, apart from the most apical cells.
conclusions. Uroplakin Ib is highly expressed in ocular surface epithelia. As in
bladder epithelium, uroplakin Ib may protect the ocular surface from
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