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Lilley Leong, A. Sue Menko, Gerald B. Grunwald; Differential Expression of N- and B-Cadherin during Lens Development. Invest. Ophthalmol. Vis. Sci. 2000;41(11):3503-3510.
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purpose. To analyze the dynamics of N- and B-cadherin cell adhesion molecule
expression and cytoskeletal interaction during embryonic chick lens
methods. Localization of N- and B-cadherin, F-actin, and connexin 56 were
determined by immunohistochemistry of developing lenses or
immunocytochemistry of differentiating primary lens cultures.
Biochemical analysis of cytoskeletal linkage of N- or B-cadherin was
assessed by differential detergent extraction, electrophoresis, and
results. The results indicate that although both cadherins are expressed
throughout lens development, N-cadherin expression detected was similar
in both lens epithelial and fiber cells, whereas B-cadherin was
preferentially localized to the lens fiber cells. During
differentiation, both cadherins become increasingly associated with the
lens cytoskeleton, as indicated biochemically by a transition from
largely Triton X-100–soluble to Triton X-100–insoluble pools and
immunocytologically by cadherin localization to cell–cell borders and
colocalization with the actin cytoskeleton. Although a significant
fraction of N-cadherin remains Triton X-100–soluble as the lens cells
differentiate, B-cadherin becomes resistant to extraction by both
Triton X-100 as well as RIPA buffers. As detected immunocytochemically
in lens cell cultures, the temporal localization of N-cadherin to
cell–cell interfaces precedes that of B-cadherin. Furthermore,
temporal localization of B-cadherin, as opposed to N-cadherin, to
cell–cell borders more closely parallels that of connexin 56 in vitro
as well as in vivo.
conclusions. These results suggest that while both N- and B-cadherin are expressed
during lens cell differentiation, both their patterns of expression as
well as their cytoskeletal association differ between epithelial and
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