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José D. Ríos, Keisha Forde, Yolanda Diebold, Jessica Lightman, James D. Zieske, Darlene A. Dartt; Development of Conjunctival Goblet Cells and Their Neuroreceptor Subtype Expression. Invest. Ophthalmol. Vis. Sci. 2000;41(8):2127-2137.
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purpose. To investigate expression of muscarinic, cholinergic, and adrenergic
receptors on developing conjunctival goblet cells.
methods. Eyes were removed from rats 9 to 60 days old, fixed, and used for
microscopy. For glycoconjugate expression, sections were stained with
Alcian blue/periodic acid-Schiff’s reagent (AB/PAS) and with the
lectins Ulex europeus agglutinin I (UEA-I) and Helix pomatia agglutinin (HPA). Goblet cell
bodies were identified using anti-cytokeratin 7 (CK7). Nerve fibers
were localized using anti-protein gene product 9.5. Location of
muscarinic and adrenergic receptors was investigated using
anti-muscarinic and β-adrenergic receptors.
results. At days 9 and 13, single apical cells in conjunctival epithelium
stained with AB/PAS, UEA-I, and CK7. At days 17 and 60, increasing
numbers of goblet cells were identified by AB/PAS, UEA-I, HPA, and CK7.
Nerve fibers were localized around stratified squamous cells and at the
epithelial base at days 9 and 13, and around goblet cells and at the
epithelial base at days 17 and 60. At days 9 and 13, M2-
and M3-muscarinic and β2-adrenergic receptors
were found in stratified squamous cells, but M1-muscarinic
and β1-adrenergic receptors were not detected. At days 17
and 60, M2- and M3-muscarinic receptors were
found in goblet cells, whereas M1-muscarinic receptors were
in stratified squamous cells. β1- andβ 2-Adrenergic receptors were found on both cell types.β 3-Adrenergic receptors were not detected.
conclusions. In conjunctiva, nerves, M2- and M3-muscarinic,
and β1- and β2-adrenergic receptors are
present on developing goblet cells and could regulate secretion as
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