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Jung-Min Lim, Sangwon Byun, Seok Chung, Tai Hyun Park, Jong-Mo Seo, Choun-Ki Joo, Hum Chung, Dong-il Cho; Retinal Pigment Epithelial Cell Behavior is Modulated by Alterations in Focal Cell–Substrate Contacts. Invest. Ophthalmol. Vis. Sci. 2004;45(11):4210-4216. doi: 10.1167/iovs.03-1036.
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purpose. To investigate how the cellular behavior of cultured retinal pigment epithelial (RPE) cells was affected by the manipulation of early focal contact.
methods. To manipulate early focal contact, a reduced focal cell–substrate contact area on the micropatterned surfaces was implemented by microfabrication with polydimethylsiloxane (PDMS). The micropatterned PDMS surfaces had a circular pillar with a diameter of 5 μm. The human retinal pigment epithelial cell line, ARPE-19, was seeded onto the fibronectin-coated PDMS surfaces. Cell adhesion, growth, cell cycle, morphology, and interleukin-6 (IL-6) expression were observed for 3 weeks.
results. The fluorescent images of localized vinculin and actin stress fibers appeared to be more prominent on smooth PDMS surfaces. Although there was no significant effect on cell adhesion, a statistically significant inhibition of cell cycle progression was observed for micropatterned PDMS surfaces. Similarly, micropatterned surfaces showed significantly less cell growth than that of smooth surfaces. Cultures over a period of 3 weeks showed a distinct cell–cell phenotype discrepancy. Furthermore, IL-6 mRNA and secreted protein induced by IL-1β in ARPE-19 were downregulated on micropatterned PDMS surfaces.
conclusions. Disturbed focal contact in ARPE-19 cells grown on micropatterned surfaces altered cell cycle, growth, morphology, and the expression of IL-6 in vitro.
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