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Judith D. Ochrietor, Tatiana P. Moroz, Leslie van Ekeris, Michael F. Clamp, Stephanie C. Jefferson, Ana C. deCarvalho, James M. Fadool, Graeme Wistow, Takashi Muramatsu, Paul J. Linser; Retina-Specific Expression of 5A11/Basigin-2, a Member of the Immunoglobulin Gene Superfamily. Invest. Ophthalmol. Vis. Sci. 2003;44(9):4086-4096. doi: 10.1167/iovs.02-0995.
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purpose. 5A11/Basigin has recently been identified as a critical glycoprotein for full maturity and function of the mouse retina. However, the biological function of 5A11/Basigin has yet to be determined. Previous reports indicate the presence of multiple 5A11/Basigin polypeptides within the retina. Therefore, in an effort to determine the function of 5A11/Basigin, the molecular diversity of its expression was evaluated.
methods. Northern blot and immunoblot techniques were used to evaluate the number of forms of 5A11/Basigin in the mouse retina. cDNA cloning, using a mouse retina library or RT-PCR from rat, chicken, zebrafish, and human retina, was performed to determine the sequence of 5A11/Basigin transcripts. A peptide was generated, based on the deduced amino acid sequence, for subsequent antibody production. Localization of 5A11/Basigin expression was evaluated by immunoblot, immunohistochemistry, and real-time RT-PCR.
results. Two 5A11/Basigin transcripts of ∼1.5 kb and ∼1.8 kb, which correspond to glycosylated proteins of ∼45 and ∼55 kDa, respectively, were identified in mouse retina. The shorter form was previously cloned. However, the longer form, a splice variant of mouse 5A11/Basigin, is a member of the immunoglobulin gene superfamily and has been named 5A11/Basigin-2. Homologous transcripts were also cloned from rat, chicken, zebrafish, and human retina. 5A11/Basigin-2 expression was limited to the retina, specifically to photoreceptor cells, where it appeared to be most concentrated in the inner segments.
conclusions. The specific and limited expression of 5A11/Basigin-2 explicitly within photoreceptor cells implies that this glycoprotein plays a fundamental role within the retina. However, its role remains to be determined.
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