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Ineta Vysniauskiene, Reto Allemann, Josef Flammer, Ivan O. Haefliger; Vasoactive Responses of U46619, PGF2α, Latanoprost, and Travoprost in Isolated Porcine Ciliary Arteries. Invest. Ophthalmol. Vis. Sci. 2006;47(1):295-298. doi: 10.1167/iovs.05-0760.
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© ARVO (1962-2015); The Authors (2016-present)
purpose. To compare the vasoactive properties of the prostanoids U46619 (thromboxane A2 analogue), prostaglandin F2α (PGF2α), latanoprost free acid, and travoprost free acid in isolated porcine ciliary arteries.
methods. In a myograph system (isometric force measurement), quiescent vessels were exposed (cumulatively) to U46619, PGF2α, latanoprost, or travoprost (0.1 nM–0.1 mM). Experiments were also conducted in the presence of SQ 29548 (TP-receptor antagonist; 3–10 μM) or AL-8810 (FP-receptor antagonist; 3–30 μM). Contractions were expressed as the percentage of 100 mM potassium chloride-induced contractions.
results. In quiescent vessels, contractions (concentration–response curves) induced by (0.1 mM) PGF2α (87.9% ± 3.5%), U46619 (66.7% ± 4.1%), and latanoprost (62.9% ± 3.6%) were more pronounced (P ≤ 0.001) than those induced by travoprost (23.0% ± 4.4%). Concentration–response curves for PGF2α, latanoprost, and travoprost were preceded by a smaller contraction peak (0.1 μM) that was higher (P ≤ 0.05) for travoprost (24.4% ± 2.8%) than for PGF2α (12.9% ± 4.6%), but not different (P = 0.58) from latanoprost (22.0% ± 3.0%). The 50% maximal contraction (PD50: negative log M concentration) of U46619 (−8.05 ± 0.13) was lower (P ≤ 0.001) than those of latanoprost (−5.65 ± 0.10), PGF2α (−5.49 ± 0.14), and travoprost (−5.12 ± 0.52). Contractions were inhibited (P ≤ 0.05–0.001) either by SQ 29548 or AL-8810.
conclusions. In isolated porcine ciliary arteries, all prostanoids tested induced contractions. Among them, travoprost appeared to be the least potent and U46619 the most efficient.
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