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Yuko Yodoi, Manabu Sasahara, Takanori Kameda, Nagahisa Yoshimura, Atsushi Otani; Circulating Hematopoietic Stem Cells in Patients with Neovascular Age-Related Macular Degeneration. Invest. Ophthalmol. Vis. Sci. 2007;48(12):5464-5472. doi: 10.1167/iovs.07-0093.
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purpose. Circulating hematopoietic stem cells (HSCs) appear to have roles in the formation of choroidal neovascularization (CNV) in age-related macular degeneration (AMD). This study was conducted to investigate whether the number or function of HSCs plays a role in neovascular AMD.
methods. Eighty-one patients with neovascular AMD who underwent comprehensive fundus examinations every 3 months were included. The number of CD34+ HSCs isolated from peripheral blood was counted by flow cytometry. Serum cytokine levels were assessed by enzyme-linked immunosorbent assay. To examine the function of circulating HSCs, mononuclear cells were cultured and then colony forming unit (CFU-EC) and migration were measured.
results. The number of circulating CD34+ HSCs was significantly increased in the patients with active CNV without major systemic diseases (stable: 3.8 ± 0.3 cells/μL, active: 5.5 ± 0.7 cells/μL, stable versus active: P < 0.05). The number of HSCs correlated positively with the erythropoietin serum level (r = 0.47, P = 0.002). Although there was no significant difference in the CFU-EC between the patients with CNV and the control subjects, a significant decrease of CFU-EC was observed in the patients with bilateral or larger CNV.
conclusions. The findings suggest that CD34+ HSCs may be recruited from bone marrow through a signal from active CNV. Furthermore, HSCs may play a role in the severity of CNV.
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