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VIRGINA L. WEIMAR, KENNETH H. HARAGUCHI; The Development of Enzyme Activities in Corneal Connective Tissue Cells during the Lag Phase of Wound Repair I. 5-Nucleotidase and Succinic Dehydrogenase. Invest. Ophthalmol. Vis. Sci. 1965;4(5):853-866.
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The activities of two enzyme systems, 5-nucleotidase and succinic dehydrogenase, which do not occur in normal rat corneal connective tissue cells, have been investigated during the socalled "lag" phase of wound repair. The results clearly show that there is new enzyme formation or activation in the cells surrounding the injured area within short periods after injury to the cornea. Since the inflammatory reaction also occurs at the same time as the "lag phase," special precautions have been taken to identify unequivocally the cell types possessing enzyme activity. 5-Nucleotidase began to develop faint activity in the corneal fibrocytes within 6 hours and became progressively more intense throughout the first 4 days, becoming gradually restricted to the fibroblasts at the wound edge. Four days after the injury occurred, activity began in the basal cell layers of the epithelium adjacent to the active fibroblasts in the wound. The succinic dehydrogenase reaction in the connective tissue was very similar to that of 5-nucleotidase, but its activity increased up to 7 days after injury.
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