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CLAES H. DOHLMAN, BERNARD WORTMAN, SIW HULTMAN; Incorporation of sulfate-35S, N-acetylglucosamine-1-14C, glucose-1-14C, and galactose-1-14C into Calf and Beef Corneal Glycosaminoglycans. Invest. Ophthalmol. Vis. Sci. 1965;4(5):867-871.
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Beef corneas were incubated in vitro with N-acetylglucosamine-1-14C, glucose-1-14C, and galactose-1-14C. Calf and beef corneas were incubated in vitro with inorganic sulfate-35S. Corneal glycosaminoglycans were isolated and the mixture resolved by use of ECTEOLA anionexchanger and further fractionation with ethanol. After incubation ivith glucose-1-14C, a higher specific activity was found in keratan sulfate than in chondroitin-chondroitin sulfate, whereas the opposite was found after incubation with galactose-1-14C and sulfate-35S. Incubation of corneas with N-acetylglucosamine-1-14C resulted in approximately the same specific activity in keratan sulfate and chondroitin-chondroitin sulfate. The results are discussed in terms of the normal biosynthesis of corneal glycosaminoglycans.
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