Purchase this article with an account.
Ji-jing Pang, Sanford L. Boye, Ashok Kumar, Astra Dinculescu, Wentao Deng, Jie Li, Qiuhong Li, Asha Rani, Thomas C. Foster, Bo Chang, Norman L. Hawes, Jeffrey H. Boatright, William W. Hauswirth; AAV-Mediated Gene Therapy for Retinal Degeneration in the rd10 Mouse Containing a Recessive PDEβ Mutation. Invest. Ophthalmol. Vis. Sci. 2008;49(10):4278-4283. doi: https://doi.org/10.1167/iovs.07-1622.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. To test AAV-mediated gene therapy in the rd10 mouse, a natural model of recessive RP caused by mutation of the β-subunit of rod photoreceptor cGMP phosphodiesterase.
methods. One eye of a cohort of rd10 mice kept in a dark environment was subretinally injected at postnatal day (P) 14 with 1 μL AAV5-smCBA-PDEβ. The contralateral eye was not injected. The animals were then maintained for 2 weeks in the dark before they were moved to a normal 12-hour light/12-hour dark cycling light environment for visually guided behavioral training. Three weeks after injection, treated rd10 mice were examined by scotopic and photopic electroretinography and then killed for biochemical and morphologic examination.
results. Substantial scotopic ERG signals were maintained in treated rd10 eyes, whereas untreated eyes in the same animals showed minimal signals. Treated eyes showed photopic ERG b-wave amplitudes similar to those of the normal eyes; in untreated partner eyes, only half the normal amplitudes remained. Strong PDEβ expression was observed in photoreceptor outer segments only in treated eyes. Light microscopy showed a substantial preservation of the outer nuclear layer in most parts of the treated retina only. Electron microscopy showed good outer segment preservation only in treated eyes. A visually guided water maze behavioral test under dim light showed significantly improved performance in one eye–treated rd10 mice compared with untreated mice.
conclusions. These data demonstrate that P14 administration of AAV5-smCBA-PDEβ can prevent retinal degeneration in rd10 mice, as reflected by significant structural, biochemical, electrophysiological, and behavioral preservation/restoration. These results serve as a baseline for studying long-term retinal rescue in rd10 mice.
This PDF is available to Subscribers Only