The expression of MyHC isoforms in EOMs of small mammals share common features but demonstrate distinct differences (summarized in
Table 4). The longitudinal and cross-sectional expression patterns of slow-MyHC and 2B-MyHC appear consistent among the rabbit, rat, and mouse; both myosin isoforms are continuous along the length of EOM fibers, with no longitudinal variation.
9,10 Developmental MyHCs are expressed in the orbital layers at the proximal and distal ends but are excluded from the endplate zone of all three species. Eom-MyHC, on the other hand, is expressed at the highest levels, spanning the endplate zone.
9 –11,16,21 There are differences in expression. First, Eom-MyHC is expressed only in the orbital layer in the rat,
9,10 whereas in the rabbit, eom-MyHC is expressed at high levels in the orbital layer, less in the global layer endplate zone, and disappears from orbital fibers outside the endplate zone.
16,22,23 Eom-MyHC in mice is abundantly expressed in the orbital and global layers in the endplate zone, but only in the orbital layer in the proximal region and only in the global layer in the distal region (
Fig. 4). Second, emb-MyHC is expressed in most of the orbital fibers of the rat
9,10,21 and in almost all of the outer orbital fibers, 40% to 50% of the inner orbital fibers, and 10% to 20% of the global fibers in the rabbit.
8,16 In the mouse, it is expressed only in a subset of orbital fibers, mostly in the outer orbital layer (
Fig. 4). Third, 2A-MyHC is expressed in most of the fibers in the orbital layer and in scattered, small-diameter fast fibers in the global layer in the rat, with no longitudinal variation observed,
10,22 similar to the pattern in the rabbit.
8 However, in the mouse, 2A-MyHC is restricted to the orbital layer toward the inner orbital layer, and its expression is nearly absent from the innervational zone (
Figs. 3,
4). Fourth, in the rabbit, neo-MyHC expression is scattered across the global and inner orbital layers and has been identified in the proximal, endplate, and distal regions, with no longitudinal variation.
16 However, neo-MyHC expression in the mouse was identical with 2A-MyHC and was found exclusively in the orbital layer, not in the global layer, except in the proximal end (
Figs. 3,
4). Similarly, emb-MyHC, neo-MyHC, and 2A-MyHC were excluded from the endplate zone. The difference in myosin isoform expression among animals may reflect variations in eye movement requirements across species; however, correlation of MyHC to eye movement differences would be purely speculative.