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Michelle L. Gabriele, Hiroshi Ishikawa, Joel S. Schuman, Richard A. Bilonick, Jongsick Kim, Larry Kagemann, Gadi Wollstein; Reproducibility of Spectral-Domain Optical Coherence Tomography Total Retinal Thickness Measurements in Mice. Invest. Ophthalmol. Vis. Sci. 2010;51(12):6519-6523. doi: 10.1167/iovs.10-5662.
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© 2016 Association for Research in Vision and Ophthalmology.
To test the reproducibility of spectral-domain optical coherence tomography (SD-OCT) total retinal thickness (TRT) measurements in mice.
C57Bl/6 mice were anesthetized, and three repeated volumetric images were acquired in both eyes with SD-OCT (250 A-scans × 250 frames × 1024 samplings), centered on the optic nerve head (ONH). The mice were repositioned between scans. TRT was automatically measured within a sampling band of retinal thickness with radii of 55 to 70 pixels, centered on the ONH by using custom segmentation software. The first volumetric image acquired in a given eye was used to register the remaining two SD-OCT images by manually aligning the en face images with respect to rotation and linear translation. Linear mixed-effects models were fitted to global and quadrant thicknesses, taking into account the clustering between eyes, to assess imprecision (measurement reproducibility).
Twenty-six eyes of 13 adult mice (age 13 weeks) were imaged. The mean global TRT across all eyes was 298.21 μm, with a mouse heterogeneity standard deviation (SD) of 4.88 μm (coefficient of variation [CV] = 0.016), an eye SD of 3.32 μm (CV = 0.011), and a device-related imprecision SD of 2.33 μm (CV = 0.008). The superior quadrant had the thickest mean TRT measurement (310.38 μm) and the highest (worst) imprecision SD (3.13 μm; CV = 0.010), and the inferior quadrant had the thinnest mean TRT (291.55 μm). The quadrant with the lowest (best) imprecision SD was in the nasal one (2.06 μm; CV = 0.007).
Good reproducibility was observed for SD-OCT retinal thickness measurements in mice. SD-OCT may be useful for in vivo longitudinal studies in mice.
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