August 1969
Volume 8, Issue 4
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Articles  |   August 1969
Aqueous Flow and Entry of Cycloleucine into the Aqueous Humor of Vervet Monkeys (Cercopithecus Ethiops)
Author Affiliations
  • PER-ERIK WÁLINDER
    Department of Experimental Ophthalmology at the Wallenberg Laboratory and the Departmentof Pharmacology, University of Uppsala, Uppsala, Sweden
  • ANDERS Bill
    Department of Experimental Ophthalmology at the Wallenberg Laboratory and the Departmentof Pharmacology, University of Uppsala, Uppsala, Sweden
Investigative Ophthalmology & Visual Science August 1969, Vol.8, 434-445. doi:
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      PER-ERIK WÁLINDER, ANDERS Bill; Aqueous Flow and Entry of Cycloleucine into the Aqueous Humor of Vervet Monkeys (Cercopithecus Ethiops). Invest. Ophthalmol. Vis. Sci. 1969;8(4):434-445.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

A buffer solution containing 125I-albumin was washed to and fro through the anterior chamber of vervet monkeys given 13lI-albumin systemically. The rate of outward flow of 125I-albumin and the accumulation of 131I-albumin in the solution were determined. In 5 monkeys the concentration of 131I-albumin in the newly formed aqueous was calculated to be an average 3 per cent of that in plasma for several hours. In a sixth monkey itwas about 6 per cent after 9 hours of perfusion. The entry of 3H-cycloleucine from the blood into the anterior chamber and the rate of aqueous humor formation were then studied in vervets. 3H-cycloleucine was given parenterally; the steady-state concentration of 3H-cycloleucine in the anterior aqueous humor was 1.11 of that in plasma water. The anterior chamber was perfused with a buffer solution containing 131I-albumin or 14C-inulin. The rate of aqueous humor formation was calculated from dilution data. The flow determined with 131I-albumin in one eye was practically the same as that determined with 14C-imdin in the other eye. In experiments with 9 hours of perfusion, the rate of aqueous humor formation calculated from 14C-inulin data increased from 2.0 to 2.3 µL per minute. The simultaneously measured mean concentration of sH-cycloleucine in the anterior chamber fluid increasedfrom 14.7 to 17.4 per cent of that in plasma (perfusion rate: 22 µL per minute). With these concentrations of cycloleucine in the anterior chamber fluid, about 2/3 of the amount of cycloleucine entered the chamber with the newly formed aqueous, and 1/3 entered mainly by diffusion across the anterior surface of the iris. The rate of entry of 14C-AIB was about 2/3 of that of 3H-cycloleucine. As a rule rabbit eyes tolerated the procedure for only one hour.

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