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Yao Fu, Chunyi Shao, Xianqun Fan; Bone Marrow-Derived Endothelial Progenitor Cells for Treatment of Corneal Endothelial Dysfunction. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1016.
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To investigate the feasibility of inducing bone marrow-derived endothelial progenitor cells (BEPC) to differentiate into corneal endothelial cells (CEC) for the treatment of corneal endothelial dysfunction.
BEPC were isolated from human fetal bone marrow, and expression of Dil-Ac-LDL, UEA-1, CD133 and CD34 were examined to identify the cells. BEPC were co-cultured with CEC for 10 days in a transwell system with conditioned medium from CEC, and then cell transdifferentiation was examined by immunocytofluorescence and electron microscopy. With a porcine corneal acellular matrix as the carrier, the induced BEPC were transplanted onto a cat’s cornea from which Descemet’s membrane and the endothelium had been stripped.
The induced BEPC resembled CEC in polygonal shape, expressing aquaporin-1, tightly opposed cell junctions, and neurone-specific enolase. Twenty-eight days after transplantation, the transparency gradually returned to the corneas transplanted with the induced BEPC on porcine corneal acellular matrix .
Human fetal BEPC could be induced into corneal endothelial-like cells in vitro. Features of the induced BEPC indicated that they may be useful for the treatment of corneal endothelial dysfunction.
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