June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Bone Marrow-Derived Endothelial Progenitor Cells for Treatment of Corneal Endothelial Dysfunction
Author Affiliations & Notes
  • Yao Fu
    Department of Ophthalmology, Ninth People’s Hospital, Medical School of Shanghai Jiaotong University, Shanghai, China
  • Chunyi Shao
    Department of Ophthalmology, Ninth People’s Hospital, Medical School of Shanghai Jiaotong University, Shanghai, China
  • Xianqun Fan
    Department of Ophthalmology, Ninth People’s Hospital, Medical School of Shanghai Jiaotong University, Shanghai, China
  • Footnotes
    Commercial Relationships Yao Fu, None; Chunyi Shao, None; Xianqun Fan, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 1016. doi:
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    • Get Citation

      Yao Fu, Chunyi Shao, Xianqun Fan; Bone Marrow-Derived Endothelial Progenitor Cells for Treatment of Corneal Endothelial Dysfunction. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1016.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To investigate the feasibility of inducing bone marrow-derived endothelial progenitor cells (BEPC) to differentiate into corneal endothelial cells (CEC) for the treatment of corneal endothelial dysfunction.

Methods: BEPC were isolated from human fetal bone marrow, and expression of Dil-Ac-LDL, UEA-1, CD133 and CD34 were examined to identify the cells. BEPC were co-cultured with CEC for 10 days in a transwell system with conditioned medium from CEC, and then cell transdifferentiation was examined by immunocytofluorescence and electron microscopy. With a porcine corneal acellular matrix as the carrier, the induced BEPC were transplanted onto a cat’s cornea from which Descemet’s membrane and the endothelium had been stripped.

Results: The induced BEPC resembled CEC in polygonal shape, expressing aquaporin-1, tightly opposed cell junctions, and neurone-specific enolase. Twenty-eight days after transplantation, the transparency gradually returned to the corneas transplanted with the induced BEPC on porcine corneal acellular matrix .

Conclusions: Human fetal BEPC could be induced into corneal endothelial-like cells in vitro. Features of the induced BEPC indicated that they may be useful for the treatment of corneal endothelial dysfunction.

Keywords: 481 cornea: endothelium • 500 differentiation • 741 transplantation  
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