June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
The Regeneration Potential of Mouse Lacrimal Gland Following Duct Ligation Procedure
Author Affiliations & Notes
  • Ying Liu
    Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Tetsuya Kawakita
    Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Machiko Sugiyama
    Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Masatoshi Hirayama
    Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Motoko Kawashima
    Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Yoko Ogawa
    Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Masataka Ito
    National Defense Medicine College, Saitama, Japan
  • Shigeto Shimmura
    Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Kazuo Tsubota
    Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Footnotes
    Commercial Relationships Ying Liu, None; Tetsuya Kawakita, None; Machiko Sugiyama, None; Masatoshi Hirayama, None; Motoko Kawashima, Santen Pharmaceutical Co., Ltd (F); Yoko Ogawa, None; Masataka Ito, None; Shigeto Shimmura, None; Kazuo Tsubota, AcuFocus, Inc (C), Allergan (F), Bausch Lomb Surgical (C), Functional visual acuity meter (P), JiNS (P), Kissei (F), Kowa (F), Santen, Inc. (F), Otsuka (F), Pfizer (C), Thea (C), Echo Denki (P), Nidek (F), Ophtecs (F), Wakasa Seikatsu (F), CEPT Company (P)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 1022. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Ying Liu, Tetsuya Kawakita, Machiko Sugiyama, Masatoshi Hirayama, Motoko Kawashima, Yoko Ogawa, Masataka Ito, Shigeto Shimmura, Kazuo Tsubota; The Regeneration Potential of Mouse Lacrimal Gland Following Duct Ligation Procedure. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1022.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: To observe the regeneration and proliferation potential of adult mouse lacrimal gland (LG) following duct-ligation (DL) procedure and duct-ligation-release (DLR) procedure.

Methods: Adult mice were divided into two groups. One group was subjected to the DL of the right LG, and glands were collected at day 0, 3, 7 and 14 after DL (n=3-5, at each time point). Another group was subjected to the DLR for 7 days and the ligation was released at day 7 of the right LG. Then the glands were collected at day 9, 12, 14 and 17 after the ligation was released. Tear production and the gland weight were measured during the duct ligation / release (DL/R) procedure. Tissues were investigated by Hematein & Eosin (H&E) staining and immunohistochemistry.

Results: Tear secretion of DL and DLR group at day 3, 7, 9, 12 and 14, has significantly decreased compared with control (p<0.01, at each time point). After DL/R the LG went through atrophy and the weight of LG DL group at day 14, has significantly decreased compared with control (p<0.01). And DLR group at day 9, 10 and day 14 has significantly decreased compared with control (p<0.01, p<0.05, p<0.01, respectively). The H&E staining showed the regular acinar unit structures could not be observed, and the lumen of ducts were expanded accompanied with the duct unit increased in the later stage of DL/R. The immunohistochemistry of CD45 (a marker of common lymphocyte) positive cells were increased after DL/R and the immunohistochemistry of nestin (a marker of neuron stem cell) showed the nestin-positive cells in both two groups.

Conclusions: Adult mouse LG has a strong regeneration potency after atrophy as a result of inflammation.

Keywords: 576 lacrimal gland • 486 cornea: tears/tear film/dry eye • 721 stem cells  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×