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Berthold Seitz, Tanja Stachon, Jiong Wang, Nora Szentmáry; Secretion of IL-1α, IL-1β, IL-6 and IL-8 in human keratocyte cell cultures following photodynamic inactivation (PDI). Invest. Ophthalmol. Vis. Sci. 2013;54(15):103.
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Purpose: With the increasing resistance of microorganisms to antibiotics, photodynamic inactivation (PDI) may be an alternative treatment option of infectious keratitis. In previous studies we analyzed viability, apoptosis, proliferation, CD34 and alpha-smooth actin expression of human keratocyte cell cultures after PDI using the photosensitizer chlorin e6 (Ce6). The purpose of this study was to determine the secretion of various interleukins by human keratocytes following PDI, in vitro.
Methods: Primary human keratocytes were isolated by digestion in collagenase A (1 mg/ml) from human corneal buttons, and cultured in DMEM/Ham’s F12 medium supplemented with 10% FCS. Using 100 nM Ce6, twenty-four hours following illumination for 13 minutes (670 nm) the release of IL-1α, IL-1β, IL-6 and IL-8 was determined using enzyme-linked immunosorbent assay (ELISA).
Results: Twenty-four hours after PDI, IL-1α (0 to 0 pg/µg protein), IL-1β (0.19 to 0.21 pg/µg protein), IL-6 (1.17 to 1.18 pg/µg protein) and IL-8 (6.04 to 4.59 pg/µg protein) secretion did not differ significantly from untreated controls (p>0.05).
Conclusions: Photodynamic inactivation does not have an impact on secretion of IL-1α, IL-1β, IL-6 and IL-8, twenty-four hours after the treatment.
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