June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Secretion of IL-1α, IL-1β, IL-6 and IL-8 in human keratocyte cell cultures following photodynamic inactivation (PDI)
Author Affiliations & Notes
  • Berthold Seitz
    Department of Ophthalmology, Saarland University Medical Center, Homburg/Saar, Germany
  • Tanja Stachon
    Department of Ophthalmology, Saarland University Medical Center, Homburg/Saar, Germany
  • Jiong Wang
    Department of Ophthalmology, Saarland University Medical Center, Homburg/Saar, Germany
    Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan, China
  • Nora Szentmáry
    Department of Ophthalmology, Saarland University Medical Center, Homburg/Saar, Germany
  • Footnotes
    Commercial Relationships Berthold Seitz, None; Tanja Stachon, None; Jiong Wang, None; Nora Szentmáry, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 103. doi:
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      Berthold Seitz, Tanja Stachon, Jiong Wang, Nora Szentmáry; Secretion of IL-1α, IL-1β, IL-6 and IL-8 in human keratocyte cell cultures following photodynamic inactivation (PDI). Invest. Ophthalmol. Vis. Sci. 2013;54(15):103.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Purpose: With the increasing resistance of microorganisms to antibiotics, photodynamic inactivation (PDI) may be an alternative treatment option of infectious keratitis. In previous studies we analyzed viability, apoptosis, proliferation, CD34 and alpha-smooth actin expression of human keratocyte cell cultures after PDI using the photosensitizer chlorin e6 (Ce6). The purpose of this study was to determine the secretion of various interleukins by human keratocytes following PDI, in vitro.

Methods: Methods: Primary human keratocytes were isolated by digestion in collagenase A (1 mg/ml) from human corneal buttons, and cultured in DMEM/Ham’s F12 medium supplemented with 10% FCS. Using 100 nM Ce6, twenty-four hours following illumination for 13 minutes (670 nm) the release of IL-1α, IL-1β, IL-6 and IL-8 was determined using enzyme-linked immunosorbent assay (ELISA).

Results: Results: Twenty-four hours after PDI, IL-1α (0 to 0 pg/µg protein), IL-1β (0.19 to 0.21 pg/µg protein), IL-6 (1.17 to 1.18 pg/µg protein) and IL-8 (6.04 to 4.59 pg/µg protein) secretion did not differ significantly from untreated controls (p>0.05).

Conclusions: Conclusions: Photodynamic inactivation does not have an impact on secretion of IL-1α, IL-1β, IL-6 and IL-8, twenty-four hours after the treatment.

Keywords: 647 photodynamic therapy • 484 cornea: stroma and keratocytes • 490 cytokines/chemokines  
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