June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Combining holographic stimulation with cellular resolution imaging in the rodent eye
Author Affiliations & Notes
  • Shy Shoham
    Biomedical Engineering, Technion, IIT, Haifa, Israel
  • Adi Schejter
    Biomedical Engineering, Technion, IIT, Haifa, Israel
  • Limor Tsur
    Biomedical Engineering, Technion, IIT, Haifa, Israel
  • Inna Reutsky-Gefen
    Biomedical Engineering, Technion, IIT, Haifa, Israel
    Medical Engineering, Ruppin Academic Center, Emek Hefer, Israel
  • Nairouz Farah
    Biomedical Engineering, Technion, IIT, Haifa, Israel
  • Footnotes
    Commercial Relationships Shy Shoham, None; Adi Schejter, None; Limor Tsur, None; Inna Reutsky-Gefen, None; Nairouz Farah, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 1039. doi:
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    • Get Citation

      Shy Shoham, Adi Schejter, Limor Tsur, Inna Reutsky-Gefen, Nairouz Farah; Combining holographic stimulation with cellular resolution imaging in the rodent eye. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1039.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

Optical retinal prostheses for patients with outer-retinal degenerative diseases could interface directly with surviving retinal neurons in order to mimic the normal input obtained from photoreceptors in healthy retinas. Recently, we introduced an artificial photo-stimulation technique based on the projection of holographic patterns with high spatio-temporal resolution onto optogentic probes, to selectively control large retinal neuronal populations in the isolated retina. Here, we explore the ability to target single optogenetic-expressing retinal ganglion cells (RGCs) with holographic patterns at a cellular resolution, in-vivo.

 
Methods
 

For image-guided neuronal targeting, we constructed a system that combines precise spatiotemporal holographic photo-stimulation with high resolution fundus imaging. The system is also integrated with a multiphoton microscope to enable functional imaging of the responses to artificial stimulation.

 
Results
 

The system was utilized to acquire both brightfield and fluorescence fundus images of mice and rats in-vivo, enabling the identification of single fluorescent RGCs for stimulation. Holographic patterns were projected onto the rodents’ retinas and imaged. The stimulation spot diameter is sufficient for cellular targeting using patterned photo-stimulation.

 
Conclusions
 

Our system enables single-cell resolved patterned holographic photo-stimulation of RGCs in-vivo which will enable the further development of a novel optical retinal prosthesis.

 
Keywords: 531 ganglion cells • 551 imaging/image analysis: non-clinical • 688 retina  
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