June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Evaluation of drug delivery and biocompatibility of biodegradable microfilms in the anterior segment of the rat model
Author Affiliations & Notes
  • Yan Peng
    School of Materials Science and Engineering, Nanyang Technological University, Singapore, Singapore
  • Yu-Chi Liu
    Singapore Eye Research Institute, Singapore, Singapore
    Singapore National Eye Centre, Singapore, Singapore
  • Nyein Chan Lwin
    Singapore Eye Research Institute, Singapore, Singapore
  • Subbu S Venkatraman
    School of Materials Science and Engineering, Nanyang Technological University, Singapore, Singapore
  • Tina Wong
    Singapore Eye Research Institute, Singapore, Singapore
    Singapore National Eye Centre, Singapore, Singapore
  • Jodhbir Mehta
    Singapore Eye Research Institute, Singapore, Singapore
    Singapore National Eye Centre, Singapore, Singapore
  • Footnotes
    Commercial Relationships Yan Peng, None; Yu-Chi Liu, None; Nyein Chan Lwin, None; Subbu S Venkatraman, None; Tina Wong, 61, 250,006 (P); Jodhbir Mehta, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 1092. doi:
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      Yan Peng, Yu-Chi Liu, Nyein Chan Lwin, Subbu S Venkatraman, Tina Wong, Jodhbir Mehta; Evaluation of drug delivery and biocompatibility of biodegradable microfilms in the anterior segment of the rat model. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1092.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To develop a biodegradable, sustained-release, prednisolone acetate (PA)-loaded poly [d,l-lactide-co-ε-caprolactone] (PLC) drug delivery system and to evaluate its biocompatibility, feasibility and release characteristics both in vitro and in vivo.

Methods: Blank and 40% PA-loaded PLC microfilms with thickness of 100µm and diameter of 2mm were developed and tested in vitro and in vivo. The degradation and drug release profiles of the microfilms were evaluated in the in vitro and in vivo experiments. We further implanted the microfilms to the subconjunctival space of the rats (n=51). All eyes were monitored using slit lamp bio-microscopy with Hackett McDonald ocular scoring system and anterior segment optical coherence tomography. Histological studies with Hematoxylin-Eosin, Picrosirus red staining and immunohistochemistry analysis were performed to evaluate and compare the presence of inflammatory and fibrotic reaction in blank and PA-loaded microfilm groups. PA concentrations in the aqueous humor were determined by high-performance liquid chromatography (HPLC).

Results: Subconjunctivally-implanted PA-loaded PLC microfilms were able to deliver prednisolone acetate in a sustained manner over 3 months, with a steady rate of 0.002mg/day in vivo. Eyes with either blank or PA-loaded implanted microfilms showed very minimal inflammatory response at the insertion sites and mild degree of collagen encapsulation around the microfilms, with significantly less CD11c cells at 2 weeks (P = 0.001) and collagen extent at 2 and 4 weeks (P = 0.001 and P = 0.002) in PA-loaded microfilm group. Desirable anterior chamber PA levels were achieved, with the concentrations at 76.67±5.86, 69.33±2.3 and 42.67±4.1 ng/ml at 2, 4 and 12 weeks, respectively.

Conclusions: PA-loaded PLC microfilm displays good biocompatibility, feasibility and desirable sustained drug release profiles. This device provides a promising alternative with great potential application in the treatments of ocular anterior segment diseases.

Keywords: 421 anterior segment • 487 corticosteroids • 497 development  
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