June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Polyunsaturated fatty acids and Plasmalogens in diabetics
Author Affiliations & Notes
  • Philippe Koehrer
    Department of Ophthalmology, University Hospital, Dijon, France
  • Niyazi Acar
    Eye and Nutrition Research Group, UMR CSGA-1324 INRA-6265 CNRS-Université de Bourgogne-AgroSup, Dijon, France
  • Sarah Saab
    Eye and Nutrition Research Group, UMR CSGA-1324 INRA-6265 CNRS-Université de Bourgogne-AgroSup, Dijon, France
  • Rodica Isaico
    Department of Ophthalmology, University Hospital, Dijon, France
  • Alain Bron
    Department of Ophthalmology, University Hospital, Dijon, France
  • Lionel Bretillon
    Eye and Nutrition Research Group, UMR CSGA-1324 INRA-6265 CNRS-Université de Bourgogne-AgroSup, Dijon, France
  • Catherine Garcher
    Department of Ophthalmology, University Hospital, Dijon, France
  • Footnotes
    Commercial Relationships Philippe Koehrer, None; Niyazi Acar, None; Sarah Saab, None; Rodica Isaico, None; Alain Bron, Allergan (C), Bausch Lomb (C), Horus (F), Théa (C); Lionel Bretillon, None; Catherine Garcher, Alcon (C), Allergan (C), Baush and Lomb (C), Bayer Pharma (C), Novartis (C), Laboratoire Théa (C)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 1158. doi:
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    • Get Citation

      Philippe Koehrer, Niyazi Acar, Sarah Saab, Rodica Isaico, Alain Bron, Lionel Bretillon, Catherine Garcher; Polyunsaturated fatty acids and Plasmalogens in diabetics. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1158.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

Plasmalogens are phospholipids characterized by a vinyl ether bond and a preferential esterification of polyunsaturated fatty acids (PUFA). We have previously shown that the lack of plasmalogens leads to retinal hyper-capillarization. We hypothesize that plasmalogens are negative regulators of vascular development, and aimed to check their circulating levels in diabetic patients with a retinopathy.

 
Methods
 

Blood samples were collected from 88 patients and 14 control subjects. Among diabetics we had 14 patients without diabetic retinopathy (DR), 12 with a mild non proliferative DR, 12 with a moderate non proliferative DR, 22 with a severe non proliferative DR and 24 with a proliferative DR (PDR). Erythrocytes were isolated from total blood samples. The plasmalogen content and the fatty acid composition of erythrocyte phospholipids were determined using capillary column gas chromatography. Individual species of phospholipids, including plasmalogens, were quantified by liquid chromatography coupled with a triple quadrupole mass spectrometry instrument (HPLC-ESI+MS/MS).

 
Results
 

Gas chromatographic analyzes showed significant reduced levels of omega-3 PUFAs including docosahexaenoic acid (DHA) (p<0.01) and omega-6 (p=0.01) PUFAs including arachidonic acid in diabetics without DR and with DR at any stage vs controls. The ratio omega-6/omega-3 tended to increase to become significant in PDR patients vs controls (p=0.01). The analysis of individual species of phospholipids by HPLC-ESI+MS/MS showed reduced levels of choline-phospholipids esterified with DHA in PRD patients vs controls (p=0.02) and reduced levels of ethanolamine-phospholipids esterified with DHA at the mild, moderate, severe stages (p=0.03) vs controls. Levels of cholin and ethanolamine-plasmalogens remained stable in all the diabetics when compared to controls.

 
Conclusions
 

The changes observed for omega-3 and omega-6 PUFA between controls and diabetics deserve further studies.

 
Keywords: 499 diabetic retinopathy • 583 lipids  
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