June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Transplanted Olfactory Ensheathing Cells Reduce Retinal Degeneration in a rat model of retinitis pigmentosa
Author Affiliations & Notes
  • Zheng Qin Yin
    Department of Ophthalmology, Southwest Hospital, Chongqing, China
  • Shu Jia Huo
    Department of Ophthalmology, Southwest Hospital, Chongqing, China
  • Yao Chen Li
    Department of Ophthalmology, Southwest Hospital, Chongqing, China
  • Jing Xie
    Department of Ophthalmology, Southwest Hospital, Chongqing, China
  • Footnotes
    Commercial Relationships Zheng Qin Yin, None; Shu Jia Huo, None; Yao Chen Li, None; Jing Xie, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 1174. doi:
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      Zheng Qin Yin, Shu Jia Huo, Yao Chen Li, Jing Xie; Transplanted Olfactory Ensheathing Cells Reduce Retinal Degeneration in a rat model of retinitis pigmentosa. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1174.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Retinitis pigmentosa (RP) is a group of genetic disorders and a slow loss of vision that is caused by a cascade of retinal degenerative events. We examined whether these retinal degenerative events were reduced after cultured mixtures of adult olfactory ensheathing cells (OECs) and olfactory nerve fibroblasts (ONFs), were transplanted into the subretinal space of 1-month-old RCS rat, a classic model of RP.

Methods: The changes in retinal photoreceptors and Müller cells of RCS rats after cell transplantation were observed by the expression of recoverin and glial fibrillary acidic protein (GFAP), counting peanut agglutinin (PNA)-positive cone outer segments and calculating the relative apoptotic area. The retinal function was also evaluated by Flash electroretinography (ERG). To further investigate the mechanisms, by which OECs/ONFs play important roles in the transplanted retinas, nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and basic fibroblast growth factor (bFGF) secretion of the cultured cells were analyzed by ELISA. The ability of OECs/ONFs to ingest porcine retinal outer segments and the amount of phagocytosis were compared with retinal pigment epithelium (RPE) cells.

Results: Our research showed that the transplantation of OECs/ONFs mixtures restored recoverin expression, protected retinal outer segments, increased PNA-positive cone outer segments reduced caspase-positive apoptotic figures, downregulated GFAP, and maintained the b-wave of the ERG. Cultured OECs/ONFs expressed and secreted NGF, BDNF, and bFGF which made contributions to assist survival of the photoreceptors. An in vitro phagocytosis assay showed that OECs, but not ONFs, phagocytosed porcine retinal outer segments, and the phagocytic ability of OECs were even superior to that of RPE cells.

Conclusions: These findings demonstrate that transplantation of OECs/ONFs cleaned up the accumulated debris in subretinal space, and provided an intrinsic continuous supply of neurotrophic factors, and suppressed the gliotic response of the Müller cells. It suggested that transplantation of OECs/ONFs might be a possible future route for protection of the retina and reducing retinal degeneration in RP.

Keywords: 695 retinal degenerations: cell biology • 648 photoreceptors • 615 neuroprotection  
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