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Dong Hyun Jo, Dain Son, Yirang Na, Manyoung Jang, Jae-Hoon Choi, Jin Hyoung Kim, Young Yu, Seung Hyeok Seok, Jeong Hun Kim; An Orthotopic Transplantation Model of Retinoblastoma in Zebrafish: A Novel Gateway for Screening of Anticancer Drugs. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1254.
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© ARVO (1962-2015); The Authors (2016-present)
To establish a novel orthotopic transplantation model of retinoblastoma in zebrafish for efficient screening of anticancer drugs
We injected differential numbers (20 and 100) of retinoblastoma cells into the vitreous cavity of zebrafish at 48 hours after fertilization. Eyeballs of zebrafish were scanned daily under the confocal laser microscope. Captured images were used for quantitative analysis with the public image processing program, ImageJ. Transplanted retinoblastoma cells were isolated to perform further analyses including Western blotting for glial fibrillary acidic protein and neuron-specific enolase, reverse transcriptase for cellular retinaldehyde-binding protein, to confirm that retinoblastoma cells maintained their characteristics as tumor cells even with transplantation and isolation. To figure out the potential of this model for screening of anticancer drugs, zebrafish were treated with carboplatin and melphalan systemically after the injection of retinoblastoma cells.
The degree of the tumor population estimated by the mean intensity of GFP expression was dependent on the number of retinoblastoma cells injected. The tumor population maintained stably for at least 4 days after the injection. Transplanted retinoblastoma cells maintain the proliferative potential and characteristics as retinoblastoma cells after isolation. Interestingly, systemic application of carboplatin and melphalan induced significant reduction in the tumor population, which could be quantitatively analyzed by the estimation of the mean intensity of GFP expression.
This orthotopic transplantation model of retinoblastoma in zebrafish is expected to be utilized for the screening of anticancer drugs for the treatment of retinoblastoma.
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