June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Heme oxygenase-1 gene therapy in a mice model of atrophic age-related macular degeneration
Author Affiliations & Notes
  • Ming-Hui Sun
    Ophthalmology, Chang Gung Memorial Hospital -LinKou, Kwei-Shan, Tao-Yuan, Taiwan
  • Yeou-Ping Tsao
    ophthalmology, Chang Gung Memorial Hospital, Taoyuan, Taiwan
  • Kuan-Jen Chen
    Ophthalmology, Chang Gung Memorial Hospital -LinKou, Kwei-Shan, Tao-Yuan, Taiwan
  • Footnotes
    Commercial Relationships Ming-Hui Sun, None; Yeou-Ping Tsao, None; Kuan-Jen Chen, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 1376. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Ming-Hui Sun, Yeou-Ping Tsao, Kuan-Jen Chen; Heme oxygenase-1 gene therapy in a mice model of atrophic age-related macular degeneration. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1376.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract
 
Purpose
 

to study the protective effect of heme-oxygenase-1 (HO-1) in a mice model of atrophic age-related macular degeneration (AMD) by gene therapy

 
Methods
 

Two-month-old C57BL/6J mice were received subretinal injection of AAV-HO-1 or AAV-LacZ. Three weeks later, mice were immunized by peritoneal injection of carboxyethylpyrrole-mouse serum albumin (CEP-MSA) in complete Freund’s adjuvant (CFA) at day 0, followed by challenge with CEP-MSA in incomplete Freund’s adjuvant (IFA) at day 10, and challenged with CEP-MSA in CFA at 10 days before sacrifice. Three months after immunization, the mice were taken fundus pictures before sacrifice. Immunohistochemistry was performed to study the localization and expression of complement C3d. Morphology assessment of retina tissues were observed by standard microscope through hematoxylin-eosin (H&E) staining

 
Results
 

geographic retinal atrophy, significant increase in thickness of bruch membrane, and deposits of complement C3d were observed in control mice and in mice injected with AAV-LacZ 3 months after immunization of CEP. Mice injected with HO-1had less retinal degeneration, preserved the structure of retinal pigment epithelium (RPE) and bruch membrane, and attenuated the deposits of complement C3d in bruch membrane.

 
Conclusions
 

HO-1 gene therapy conferred protection on RPE in a mice model of atrophic AMD through immunized by CEP.

 
 
histology of retina (H&E staining)
 
histology of retina (H&E staining)
 
 
Immunofluorescence for expression of complement component C3d
 
Immunofluorescence for expression of complement component C3d
 
Keywords: 538 gene transfer/gene therapy • 412 age-related macular degeneration • 438 Bruch's membrane  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×