June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Nmnat3 inhibits up-regulation of p62 in high IOP-induced optic nerve degeneration
Author Affiliations & Notes
  • Yasushi Kitaoka
    Ophthalmology, St. Marianna University School of Medicine, Kawasaki, Japan
  • Yasunari Munemasa
    Ophthalmology, St. Marianna University School of Medicine, Kawasaki, Japan
  • Kaori Kojima
    Ophthalmology, St. Marianna University School of Medicine, Kawasaki, Japan
  • Satoki Ueno
    Ophthalmology, St. Marianna University School of Medicine, Kawasaki, Japan
  • Footnotes
    Commercial Relationships Yasushi Kitaoka, Kowa Company, Ltd. (F), Kowa Company, Ltd. (C); Yasunari Munemasa, None; Kaori Kojima, None; Satoki Ueno, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 1412. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Yasushi Kitaoka, Yasunari Munemasa, Kaori Kojima, Satoki Ueno; Nmnat3 inhibits up-regulation of p62 in high IOP-induced optic nerve degeneration. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1412.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: To examine the relationship of p62 and Nmnat3 in high IOP-induced optic nerve degeneration.

Methods: Eight-week-old male Wistar rats were received the combination of injection of india ink and laser treatment to trabecular meshwork. Transfection of Nmnat3 was performed with electroporation after intravitreal injection of DNA plasmid. Intravitreal injection of rapamycin (2 μl; 1 mM) was also performed. The expression of p62 in optic nerve was examined by immunoblotting. Localization of p62 and Nmnat3 in RGC-5 cell was evaluated by immunocytochemistry.

Results: Immunoblotting showed that there was an increase in p62 protein level in optic nerve after IOP elevation compared to the control group and this increase was significantly inhibited by Nmnat3 transfection. Nmnat3 transfection alone significantly decreased p62 protein levels in the optic nerve compared with the basal level. Immunocytochemistry showed that p62 was colocalized with MitoTracker Red in starved RGC-5 cells. Substantial colocalization of Nmnat3 and p62 was observed in starved RGC-5 cells.

Conclusions: p62 was present in mitochondria in starved RGC-5 cells. Mitochondrial Nmnat3 inhibits up-regulation of p62 in optic nerve in a hypertensive glaucoma rat model.

Keywords: 613 neuro-ophthalmology: optic nerve • 615 neuroprotection • 629 optic nerve  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×