June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Induction of autophagy prevents increase of p-PS1 in TNF-induced optic nerve axonal degeneration
Author Affiliations & Notes
  • Kaori Kojima
    Ophthalmology, St Marianna Univ School of Med, Kawasaki, Japan
  • Yasushi Kitaoka
    Ophthalmology, St Marianna Univ School of Med, Kawasaki, Japan
  • Yasunari Munemasa
    Ophthalmology, St Marianna Univ School of Med, Kawasaki, Japan
  • Satoki Ueno
    Ophthalmology, St Marianna Univ School of Med, Kawasaki, Japan
  • Footnotes
    Commercial Relationships Kaori Kojima, None; Yasushi Kitaoka, Kowa Company, Ltd. (F), Kowa Company, Ltd. (C); Yasunari Munemasa, None; Satoki Ueno, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 1419. doi:
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    • Get Citation

      Kaori Kojima, Yasushi Kitaoka, Yasunari Munemasa, Satoki Ueno; Induction of autophagy prevents increase of p-PS1 in TNF-induced optic nerve axonal degeneration. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1419.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: We previously reported that an increase in phosphorylated presenilin 1 (p-PS1) in optic nerve may be associated with TNF-induced axonal degeneration. In this study, we investigated the involvement of autophagy in this condition.

Methods: Eight-week-old Wistar rats were received intravitreal injection of 10ng TNF alone or simultaneous injection of TNF and rapamycin into the right eyes. PBS was injected contralateral left eyes as control. Eyes were obtained 1 and 2 weeks after intravitreal injection. Levels of p-PS1 protein in optic nerve were determined by Western blot analysis. The effect of rapamycin on TNF-induced axonal degeneration was examined with optic nerve cross sections stained with 1% paraphenylene-diamine. The quantification was performed with Aphelion software.

Results: Western blot analysis showed increases in p-PS1 protein levels in optic nerve after TNF injection compared with those after PBS injection and that these increases were significantly prevented by rapamycin treatment. Morphometric analysis showed that rapamycin ameliorated axon loss induced by TNF.

Conclusions: These results suggest that induction of autophagy may be protective against TNF-induced optic nerve degeneration with inhibition of p-PS1.

Keywords: 613 neuro-ophthalmology: optic nerve • 615 neuroprotection • 629 optic nerve  
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