June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Functional Optical Coherence Tomography with a Commercial Device - A Pilot Study
Author Affiliations & Notes
  • Michel Teussink
    Ophthalmology, Radboud University Nijmegen Medical Centre, Nijmegen, Netherlands
  • Mark J J P van Grinsven
    Radiology, Radboud University Nijmegen Medical Centre, Nijmegen, Netherlands
  • Ramon van Huet
    Ophthalmology, Radboud University Nijmegen Medical Centre, Nijmegen, Netherlands
  • Barry Cense
    Center of Optical Research and Education, Utsunomiya University, Utsunomiya, Japan
  • Carel Hoyng
    Ophthalmology, Radboud University Nijmegen Medical Centre, Nijmegen, Netherlands
  • B. Jeroen Klevering
    Ophthalmology, Radboud University Nijmegen Medical Centre, Nijmegen, Netherlands
  • Thomas Theelen
    Ophthalmology, Radboud University Nijmegen Medical Centre, Nijmegen, Netherlands
  • Footnotes
    Commercial Relationships Michel Teussink, None; Mark J J P van Grinsven, None; Ramon van Huet, None; Barry Cense, Topcon (F), US2007-0038040 (P), US2012-0038885 (P), JP2012-064565 (P), JP2012-067488 (P); Carel Hoyng, None; B. Jeroen Klevering, None; Thomas Theelen, Heidelberg Engineering (F), Heidelberg Engineering (R)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 1484. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Michel Teussink, Mark J J P van Grinsven, Ramon van Huet, Barry Cense, Carel Hoyng, B. Jeroen Klevering, Thomas Theelen; Functional Optical Coherence Tomography with a Commercial Device - A Pilot Study. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1484.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: To evaluate, reproducibility and reliability of measurements of visually evoked retinal intrinsic optical signals (IOS) in humans by a commercially available spectral-domain OCT.

Methods: We measured visually evoked IOS in 16 healthy volunteers with a Spectralis™ OCT, extended by a custom made optical stimulator. After 20 minutes of dark adaptation, a 3 mm2 area of the temporal perimacula was stimulated with a white flash of 0.1 s duration and 5.9 log scot. tld. luminance. A single-line OCT scan was recorded on-line before (baseline), during and after stimulation. From this OCT time-series we extracted IOS by calculating changes in reflectivity from baseline after application of the light stimulus. Custom software was used to compare reflectivity changes in the stimulated area to the non-stimulated area. To improve the signal-to-noise ratio, we averaged the results of three single IOS recordings of each eye.

Results: IOS of the stimulated area could repeatedly be recorded with reproducible results. Axial motion of the OCT scans during the measurements caused substantial noise which could significantly be reduced by averaging.

Conclusions: We were able to reproducibly record visually evoked IOS with the Spectralis™ OCT coupled to an optical stimulator. Due to the high noise level, averaging of multiple measurements is necessary to clearly identify and analyze retinal signals. IOS imaging may be improved by three-dimensional eye tracking, since axial motion artifacts seem to be of significant influence.

Keywords: 630 optical properties • 552 imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • 648 photoreceptors  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×