June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
The Correlation of Plasma Cytokines with Complement Factor H polymorphism Y402H, Choroidal Thickness and Drusen Load in Dry Age-Related Macular Degeneration
Author Affiliations & Notes
  • Sijia Cao
    Ophthal & Visual Sciences, University of British Columbia, Vancouver, BC, Canada
  • Ashley Ko
    Ophthal & Visual Sciences, University of British Columbia, Vancouver, BC, Canada
  • Aikun Wang
    Ophthal & Visual Sciences, University of British Columbia, Vancouver, BC, Canada
  • Marita Partanen
    Educational & Counselling Psychology & Special Education, University of British Columbia, Vancouver, BC, Canada
  • Andrew Merkur
    Ophthal & Visual Sciences, University of British Columbia, Vancouver, BC, Canada
  • David Albiani
    Ophthal & Visual Sciences, University of British Columbia, Vancouver, BC, Canada
  • Andrew Kirker
    Ophthal & Visual Sciences, University of British Columbia, Vancouver, BC, Canada
  • Jing Cui
    Ophthal & Visual Sciences, University of British Columbia, Vancouver, BC, Canada
  • Farzin Forooghian
    Ophthal & Visual Sciences, University of British Columbia, Vancouver, BC, Canada
  • Joanne Matsubara
    Ophthal & Visual Sciences, University of British Columbia, Vancouver, BC, Canada
  • Footnotes
    Commercial Relationships Sijia Cao, None; Ashley Ko, None; Aikun Wang, None; Marita Partanen, None; Andrew Merkur, None; David Albiani, None; Andrew Kirker, None; Jing Cui, None; Farzin Forooghian, None; Joanne Matsubara, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 157. doi:
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      Sijia Cao, Ashley Ko, Aikun Wang, Marita Partanen, Andrew Merkur, David Albiani, Andrew Kirker, Jing Cui, Farzin Forooghian, Joanne Matsubara; The Correlation of Plasma Cytokines with Complement Factor H polymorphism Y402H, Choroidal Thickness and Drusen Load in Dry Age-Related Macular Degeneration. Invest. Ophthalmol. Vis. Sci. 2013;54(15):157.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Several key components of the complement cascade are associated with the pathogenesis of age-related macular degeneration (AMD), including the Y402H variation in the gene encoding complement factor H (CFH), which confers a several-fold increased risk for developing AMD. Earlier studies, including ours, demonstrated an association between drusen, a hallmark of early AMD, and increased inflammatory cytokines in retina of postmortem eye tissues. In this study, we address the correlation between plasma cytokines and drusen load, choroidal thickness and the Y402H polymorphism in CFH gene in patients with dry AMD.

Methods: Forty-four patients with dry AMD were recruited. Drusen area and volume were determined using the spectral-domain optical coherence tomography (SD-OCT) software, and choroidal thickness was measured using enhanced depth imaging (EDI). The subjects’ blood samples were analyzed for multiple cytokines using Bio-Plex suspension assays and genotyped for the CFH Y402H polymorphism. Statistical analyses were conducted using ANCOVA and multiple regression.

Results: We found an inverse correlation between choroidal thickness and drusen load (r = -0.35, p = 0.04). Drusen load also correlated with logMAR visual acuity (r = 0.32, p = 0.04). A correlation between choroidal thickness and logMAR visual acuity was suggested (r = -0.22, p = 0.21). Patients with the high-risk CC variant had higher levels of plasma cytokines than those with TT variant (IL-1β IL-1ra, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-17, G-CSF, IFN-γ, MIP-1α, TNF-α and VEGF) (p ≤ 0.05). Patients with thinner choroids also had increased levels of MIP-1β (β = -0.35, p = 0.05). No correlation was found between the tested cytokines and drusen load.

Conclusions: The CFH Y402H genotype is associated with elevated systemic levels of inflammatory cytokines. These findings provide support for the role of inflammation in the pathogenesis of AMD. The atrophy in RPE-choroid interface may be associated with the elevated systemic inflammatory cytokines. SD-OCT can be used to assess the correlation between drusen load and choroidal thickness, both of which show a relationship with visual acuity. Therefore, the measurement of these outcomes may serve as important outcome parameters in routine clinical care and in clinical trials for dry AMD.

Keywords: 412 age-related macular degeneration • 557 inflammation • 490 cytokines/chemokines  
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