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Michael Twa, Jiasong Li, Ravi Kiran Manapuram, Floredes Menodiado, Salavat Aglyamov, Stanislav Emelianov, Kirill Larin; Corneal Biomechanical Properties after UV Cross-linking in the Rabbit. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1612.
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© ARVO (1962-2015); The Authors (2016-present)
Elasticity imaging has been applied in other areas of medicine and more recently used to characterize the structural properties of ocular tissues. An OCT-based elastography method was developed and measurements were performed in rabbit corneal tissue before and after UV-riboflavin corneal cross-linking (CXL).
Corneal elastography measurements were performed using a Phase Stabilized Swept Source Optical Coherence Elastography (PhS-SSOCE) with a sensitivity of ~10 nm along with air-pulse tissue stimulation. Surface wave propagation was measured over a 6x6mm area before and after UV-riboflavin corneal cross-linking. Tissue properties (Young's modulus, surface wave propagation speed and surface wave amplitude) were measured.
Treatment resulted in a measurable increase corneal stiffness confirmed by mechanical extensiometry (before CXL: E=1.32±0.39MPa; after CXL: E=2.34±0.91MPa). Surface wave amplitude and velocity was greatest near the excitation position (Amplitude= 993nm and Velocity=0.8±0.09m/s) and decreased from this point to the apex. Following cross-linking surface wave amplitudes decreased (141nm) and wave velocity increased (8.2±5.6 m/s).
Ocular elastography can be performed using a combination of phase-sensitive OCT and air pulse stimulation. This method can detect low amplitude tissue excitation, which can be used to quantify corneal stiffness.
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