Purchase this article with an account.
Young Joo Shin, Eui Sang Chung, Tae-Young Chung, Joon-Young Hyon, Won Ryang Wee; Stemness Characteristics of Cultured Human Corneal Endothelial Cells in Various Media. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1657.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To investigate the stemness characteristics of cultured human corneal endothelial cells (HCEC) in various media.
HCECs were cultured in media A, B, BE, and E. The morphology of cells was evaluated by inverted microscopy. Immunofluorescence staining of zo-1, nestin, GFAP and COL8A2 was performed. Cell proliferation rate was evaluated with cell counting kit -8 (CCK-8) assay.
A few cultured cells were stained with nestin and almost cells expressed COL8A2. The cell cultured in media A expressed GFAP more. Cell shape became fibroblast-like in media A and became mosaic pattern in media B and BE. Fibroblast-like cell shape in media A was recovered to be mosaic pattern in media BE. Cell proliferation rate was higher in media A and BE.
Cultured HCEC showed the stem cell markers including nestin and GFAP. The expression of GFAP was different according to media.
This PDF is available to Subscribers Only