June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Stemness Characteristics of Cultured Human Corneal Endothelial Cells in Various Media
Author Affiliations & Notes
  • Young Joo Shin
    Ophthalmology, Hallym University College of Medicine, Seoul, Republic of Korea
  • Eui Sang Chung
    Department of Ophthalmology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea
  • Tae-Young Chung
    Department of Ophthalmology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea
  • Joon-Young Hyon
    Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea
    Department of Ophthalmology, Seoul National University Bundang Hospital, Seongnam, Republic of Korea
  • Won Ryang Wee
    Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships Young Joo Shin, None; Eui Sang Chung, None; Tae-Young Chung, None; Joon-Young Hyon, None; Won Ryang Wee, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 1657. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Young Joo Shin, Eui Sang Chung, Tae-Young Chung, Joon-Young Hyon, Won Ryang Wee; Stemness Characteristics of Cultured Human Corneal Endothelial Cells in Various Media. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1657.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: To investigate the stemness characteristics of cultured human corneal endothelial cells (HCEC) in various media.

Methods: HCECs were cultured in media A, B, BE, and E. The morphology of cells was evaluated by inverted microscopy. Immunofluorescence staining of zo-1, nestin, GFAP and COL8A2 was performed. Cell proliferation rate was evaluated with cell counting kit -8 (CCK-8) assay.

Results: A few cultured cells were stained with nestin and almost cells expressed COL8A2. The cell cultured in media A expressed GFAP more. Cell shape became fibroblast-like in media A and became mosaic pattern in media B and BE. Fibroblast-like cell shape in media A was recovered to be mosaic pattern in media BE. Cell proliferation rate was higher in media A and BE.

Conclusions: Cultured HCEC showed the stem cell markers including nestin and GFAP. The expression of GFAP was different according to media.

Keywords: 481 cornea: endothelium • 721 stem cells  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×