June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
The Resting Potential of Rat`s Corneal Endothelial Cells
Author Affiliations & Notes
  • Nassim Calixto
    Ophthalmology, Federal Univ of Minas Gerais, Belo Horizonte, Brazil
  • Vinicius Oliveira
    Ophthalmology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
  • Renata Fleming
    Ophthalmology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
  • Sebastiao Cronemberger
    Ophthalmology, Federal Univ of Minas Gerais, Belo Horizonte, Brazil
  • Adalmir Dantas
    Ophthalmology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
  • Footnotes
    Commercial Relationships Nassim Calixto, None; Vinicius Oliveira, None; Renata Fleming, None; Sebastiao Cronemberger, None; Adalmir Dantas, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 1661. doi:
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    • Get Citation

      Nassim Calixto, Vinicius Oliveira, Renata Fleming, Sebastiao Cronemberger, Adalmir Dantas; The Resting Potential of Rat`s Corneal Endothelial Cells. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1661.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

The mechanism whereby the cornea maintains its transparency is not known. This study aims to register the presence and to quantify the resting potential (RP) of endothelial corneal cells. To the best of our knowledge this seems to be the first work to systematically measure and quantify the RP of endothelial cells of the cornea.

 
Methods
 

We performed 30 experiments on corneal preparations of Sprague-Dawley rats (n=30). Immediately after decapitation the eyeballs were removed and sectioned near the limbus. The corneas were transferred to a chamber and infused with Balanced Salt Solution (BSS) driven by a peristaltic pump in order to maintain the BSS flowing at a rate from 0.8 to 0.85 ml/min. The temperature in the chamber was set at 30 °C by means of a thermostatic bath. BSS is composed of (mmol/l): Na+ 160.0; Cl- 130.0; -HCO3 25.0; K+ 5.0; -H2PO4 3.0; Mg++ 1.0; Ca++ 1.0; Glucose 5.0, presenting a pH = 7.4 and 305 mOsm/Kg. The presence or absence of electrical signal was detected by recording its voltage variations through two pore electrodes delicately introduced into the cells and connected to a Grass polygraph. The statistical analysis was made by Graph Pad Prism 6.0.

 
Results
 

In the 30 experiments (30 eyes), the RP presented a mean of 40.60 with a standard deviation of 7.05 and a standard error of 1.29. The graphics below illustrate the findings.

 
Conclusions
 

Although many studies are still needed to understand the role of the endothelium in the transparency of the cornea, our results demonstrate: 1. the presence of the resting potential in the corneal endothelium cells 2. a mean of 40.60±7.05 mV for the resting potential of the rat’s corneal endothelial cells.

 
 
This graph shows the mean value at the center line, the standard deviation (SD) at the boxes, and the maximum and minimum values.
 
This graph shows the mean value at the center line, the standard deviation (SD) at the boxes, and the maximum and minimum values.
   
Keywords: 508 electrophysiology: non-clinical • 481 cornea: endothelium • 480 cornea: basic science  
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