June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Age-Dependent Differential Gene Expression in Human Corneal Endothelium
Author Affiliations & Notes
  • Cynthia Wang
    Jules Stein Eye Institute, Los Angeles, CA
  • Ricardo Frausto
    Jules Stein Eye Institute, Los Angeles, CA
  • Anthony Aldave
    Jules Stein Eye Institute, Los Angeles, CA
  • Footnotes
    Commercial Relationships Cynthia Wang, None; Ricardo Frausto, None; Anthony Aldave, Alcon (R), Allergan (R), NIH (F), Bausch + Lomb (C), Allergan (C)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 1687. doi:
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      Cynthia Wang, Ricardo Frausto, Anthony Aldave; Age-Dependent Differential Gene Expression in Human Corneal Endothelium. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1687.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To determine gene expression in the corneal endothelium of healthy children and adults to provide insight into the normal age-associated changes in corneal endothelial cell gene expression.

Methods: Total RNA was isolated from corneal endothelium obtained from four pediatric (≤11 years old) and four adult (≥53 years old) donor eye bank corneas. The RNA samples were processed and hybridized to the Affymetrix GeneChip 1.1ST array. Analysis of the raw intensity values contained within CEL files was performed using the gene expression module within the Partek Genomics Suite software. A list of the most significant differentially expressed genes between pediatric and adult corneal endothelium was obtained using a p-value and fold-change cutoff of 0.01 and 2, respectively.

Results: Principal component analysis (PCA) of our expression dataset demonstrated two distinct populations of samples in PCA1 with each being comprised of either the pediatric or adult samples, while hierarchical clustering showed a strong relationship within samples originating from the same age group. Differential gene expression analysis identified ten significantly regulated genes, of which seven encode for protein: ITGBL1, PREX2, DIO2, DLL4, C9orf131, HIST1H3A, and TNFAIP3.

Conclusions: The identification of several differentially expressed genes in pediatric and adult corneal endothelial cells suggests that changes in gene transcription continue well after birth. After validating the results of this study, we plan to investigate the role of each significantly differentially expressed gene in normal and abnormal corneal endothelial cell function.

Keywords: 481 cornea: endothelium • 533 gene/expression • 535 gene microarray  
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