June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Subretinal Implantation of Gelatin Films with Stem Cells Derived RPE in Rats
Author Affiliations & Notes
  • Yuntao Hu
    Doheny Eye Institute, Keck School of Medicine of the University of Southern California, Los Angeles, CA
    Ophthalmolgy, Peking University Third Hospital, Beijing, China
  • Kenrick Kuwahara
    Doheny Eye Institute, Keck School of Medicine of the University of Southern California, Los Angeles, CA
  • Padmaja Thomas
    Doheny Eye Institute, Keck School of Medicine of the University of Southern California, Los Angeles, CA
  • Bruno Diniz
    Doheny Eye Institute, Keck School of Medicine of the University of Southern California, Los Angeles, CA
    Retina Sector, Universidade Federal de Sao Paulo, Sao Paulo, Belize
  • Ramiro Ribeiro
    Doheny Eye Institute, Keck School of Medicine of the University of Southern California, Los Angeles, CA
    Hospital Evangelico de Curitiba, FEMPAR, Curitiba, Belize
  • Ashish Ahuja
    Doheny Eye Institute, Keck School of Medicine of the University of Southern California, Los Angeles, CA
  • Sherry Hikita
    Center for Stem Cell Biology and Engineering, University of California-Santa Barbara, Santa Barbara, CA
  • Lincoln Johnson
    Center for Stem Cell Biology and Engineering, University of California-Santa Barbara, Santa Barbara, CA
  • Biju Thomas
    Doheny Eye Institute, Keck School of Medicine of the University of Southern California, Los Angeles, CA
  • Mark Humayun
    Doheny Eye Institute, Keck School of Medicine of the University of Southern California, Los Angeles, CA
  • Footnotes
    Commercial Relationships Yuntao Hu, None; Kenrick Kuwahara, None; Padmaja Thomas, None; Bruno Diniz, None; Ramiro Ribeiro, None; Ashish Ahuja, None; Sherry Hikita, None; Lincoln Johnson, None; Biju Thomas, None; Mark Humayun, Bausch & Lomb (F), Bausch & Lomb (C), Bausch & Lomb (P), Bausch & Lomb (R), Bausch & Lomb (S), Alcon (C), Alcon (R), Iridex (P), Iridex (R), Replenish (I), Replenish (C), Replenish (R), Replenish (S), Second Sight (F), Second Sight (I), Second Sight (C), Second Sight (P), Second Sight (R), Second Sight (S), Regenerative Patch Technologies (I), Regenerative Patch Technologies (C)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 1763. doi:
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    • Get Citation

      Yuntao Hu, Kenrick Kuwahara, Padmaja Thomas, Bruno Diniz, Ramiro Ribeiro, Ashish Ahuja, Sherry Hikita, Lincoln Johnson, Biju Thomas, Mark Humayun; Subretinal Implantation of Gelatin Films with Stem Cells Derived RPE in Rats. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1763.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

Human embryonic stem cell-derived retinal pigment epithelial (hESC-RPE) cells were cultured on gelatin films and implanted into the subetinal space in rats in order to monitor post-implantation cell fate and gelatin degradation rate.

 
Methods
 

Monolayer cultures of hESC-RPE cells were cultured on 27 μm thick gelatin films. RCS rats were randomly divided into two groups and subretinally implanted with gelatin films without the cells (Group A, n=9) or gelatin films with confluent hESC-RPE cells (Group B, n=9). One week, one month and three months post-implantation OCT scanning and histological examinations were performed.

 
Results
 

The gelatin without the cells did not degrade in subretinal space up to 3 months after implantation (Fig.1). Degradation of the gelatin with hESC-RPE cells was observed at 3 months after implantation (Fig.2). However, the hESC-RPE cells maintained monolayer RPE morphology and were RPE65 positive.

 
Conclusions
 

Degradation of subretinally implanted gelatin scaffolds is cell-dependent and may be related to the release of proteolytic enzymes or other factors by hESC-RPE cells.

 
 
Fig.1 Gelatin film (without cells) did not degrade after subretinal implantation 3 months.
 
Fig.1 Gelatin film (without cells) did not degrade after subretinal implantation 3 months.
 
 
Fig.2 The hESC-RPE cells maintained monolayer on gelatin film after subretinal implantation 3 months. Gelatin degraded partly near to the hESC-RPE cells.
 
Fig.2 The hESC-RPE cells maintained monolayer on gelatin film after subretinal implantation 3 months. Gelatin degraded partly near to the hESC-RPE cells.
 
Keywords: 701 retinal pigment epithelium • 721 stem cells • 741 transplantation  
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