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LIN LU; Effect of UVB irradiation on inhibitor of apoptosis proteins(IAPs) in apoptotic retinal pigment epithelial cell lines. Invest. Ophthalmol. Vis. Sci. 2013;54(15):1769.
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Apoptosis induced by Ultraviolet radiation in the retinal pigment epithelium (RPE) cells has been implicated age-related macular degeneration(AMD). The aim of this study was to analyze expression change of inhibitor of apoptosis proteins (IAPs) in apoptotic RPE cells caused by ultraviolet B( UVB).
Cultured human retinal pigment epithelium-D407 cells(D407 RPE cells) were irradiated with UVB at different time. Then cellular morphology, cell viability, apoptosis ratios ,intracellular reactive oxygen species (ROS) production, and mitochondrial membrane potential(MMP) were evaluated. Furthermore, expression of the IAPs ( Survivin, Livin, XIAP, c-IAP1,c-IAP2,) were detected by Western blot.
UVB exposure caused significant RPE cell damage including cellular morphologic change, vitality decrease, increased apoptosis and the levels of intracellular ROS , and decreased the levels of intracellular MMP. UVB exposure also significantly decreased Expression of IAP proteins (Survivin, Livin, XIAP, c-IAP1,c-IAP2) in apoptotic D407 RPE cell.
his study showed that UVB exposure caused oxidative stress, mitochondrial dysfunction and apoptosis in retinal pigment epithelial cells in vitro. IAPs including Survivin, Livin, XIAP, c-IAP1,c-IAP2 may be inhibited in apoptotic RPE cell after UVB exposure. Prevention of UVB exposure may be useful in reducing the oxidative damage in some blinding diseases such as AMD and IAPs may be new targets for protection of RPE cell apoptosis.
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